Expression of a rice gene OsNOA1 re-establishes nitric oxide synthesis and stress-related gene expression for salt tolerance in Arabidopsis nitric oxide-associated 1 mutant Atnoa1
Expression of a rice gene OsNOA1 re-establishes nitric oxide synthesis and stress-related gene expression for salt tolerance in Arabidopsis nitric oxide-associated 1 mutant Atnoa1
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DOI：
10.1016/j.envexpbot.2008.06.002
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发表时间：
2009
影响因子：
5.7
通讯作者：
W. Qiao;Shouhua Xiao;Liang Yu;L. Fan
中科院分区：
文献类型：
--
作者：
W. Qiao;Shouhua Xiao;Liang Yu;L. Fan
一氧化氮（NO）在植物生长发育和逆境胁迫反应中具有广泛的调节作用。拟南芥基因AtNOA 1编码一种NO相关蛋白，其在耐盐性中起作用。我们采用AtNOA 1的敲除突变体，Atnoa 1是对盐敏感的，作为一种工具来评估水稻同源基因OsNOA 1的功能。OsNOA 1基因的表达挽救了正常条件下Atnoa 1的幼苗发育和营养生长，提高了Atnoa 1种子萌发、幼苗根系生长和叶绿素合成的耐盐性，降低了Atnoa 1的Na+/K+比值，NO相对含量测定表明，Atnoa 1通过OsNOA 1基因的表达重建了NO的合成;北方印迹和Semi-Q RT-PCR分析表明，OsNOA 1在Atnoa 1中的表达也重建了耐盐相关基因的表达。我们的数据表明，重新建立NO合成和耐盐相关基因的表达OsNOA 1的表达可能占Atnoa 1的发育和耐盐表型方面的恢复。上述结果提示OsNOA 1可能与AtNOA 1具有相似的作用，NO可能通过调控耐盐相关基因的表达而参与植物的耐盐过程。
Nitric oxide (NO) has been indicated in regulating a wide-spectrum of plant developmental events and stress responses. An Arabidopsis gene AtNOA1 encodes a NO-associated protein, which plays a role in salt tolerance. We employed the knockout mutant for AtNOA1, Atnoa1 that is sensitive to salinity, as a tool to evaluate the functions of a rice homologous gene, OsNOA1. OsNOA1 transgenic expression rescued Atnoa1 in seedling development and vegetative growth under normal conditions, enhanced the salt tolerance of Atnoa1 in seed germination, seedling root growth and chlorophyll synthesis, and reduced Na+/K+ratio in Atnoa1; NO relative content assay implicates that NO synthesis was re-established via OsNOA1 expression in Atnoa1; Northern blot and Semi-Q RT-PCR assays demonstrate that salt tolerance-related gene expression was re-established as well via OsNOA1 expression in Atnoa1. Our data indicate that the re-establishment of NO synthesis and salt tolerance-related gene expression by OsNOA1 expression may account for the restoration of Atnoa1 in terms of developmental and salt tolerance phenotypes. All the above results point to a notion that OsNOA1 may play similar roles as AtNOA1, and NO involvement in salt tolerance may be ascribed to its regulation of salt tolerance-related gene expression.