Pathways involved in vanadate-induced root hair formation in Arabidopsis.
Pathways involved in vanadate-induced root hair formation in Arabidopsis.
复制标题
拟南芥中钒酸盐诱导根毛形成的途径。
DOI：
10.1111/ppl.12229
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Huang HJ.
中科院分区：
文献类型：
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作者：
Lin CY;Huang LY;Chi WC;Huang TL;Kakimoto T;Tsai CR;Huang HJ.
根毛的形成受环境信号控制。我们发现，低剂量钒酸盐（V）可显著增加拟南芥根毛密度和长度。活性氧（ROS）的产生与V处理诱导。利用NADPH氧化酶抑制剂DPI和NADPH氧化酶突变体rhd 2/AtrbohC研究了V诱导的NADPH氧化酶在根系构型改变中的作用。乙烯受体（ETR 1）和根毛缺陷型（RHD 6）也参与了V诱导的根毛形成。此外，激酶抑制剂染料木黄酮（genistein（酪氨酸激酶抑制剂）和K252 a（Ser/Thr激酶抑制剂）和磷酸酶抑制剂藜芦苷（藜芦苷）。（丝氨酸/苏氨酸磷酸酶抑制剂），抑制根毛的形成诱导的V。为了阐明响应V的基因表达的调节，我们研究了基因芯片检测根中的转录变化。暴露于V引发与细胞壁形成、ROS活性和信号传导相关的基因的转录水平的变化。参与根毛形成的几个基因也受到调控。
Root hair formation is controlled by environmental signals. We found significantly increasedArabidopsisroot hair density and length in response to low‐dose vanadate (V). Reactive oxygen species (ROS) production was induced with V treatment. We investigated the possible role of NADPH oxidase in altering root system architecture induced by V by using diphenylene iodonium (DPI), an inhibitor of NADPH oxidase, and an NADPH oxidase mutant (rhd2/AtrbohC). NADPH oxidase was involved in root hair elongation induced by V. As well, ethylene receptor (ETR1) and ROOT HAIR DEFECTIVE (RHD6) participated in inducing root hair formation induced by V. Furthermore, the kinase inhibitors, genistein (tyrosine kinase inhibitor) and K252a (ser/thr kinase inhibitor), and a phosphatase inhibitor, cantharidin (ser/thr phosphatase inhibitor), suppressed root hair formation induced by V. To elucidate the regulation of gene expression in response to V, we investigated transcriptional changes in roots by microarray assay. Exposure to V triggered changes in transcript levels of genes related to cell wall formation, ROS activity and signaling. Several genes involved in root hair formation were also regulated.