Production of optically pure 2,3-butanediol from Miscanthus floridulus hydrolysate using engineered Bacillus licheniformis strains
Production of optically pure 2,3-butanediol from Miscanthus floridulus hydrolysate using engineered Bacillus licheniformis strains
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DOI：
10.1007/s11274-018-2450-7
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发表时间：
2018-04
影响因子：
4.1
通讯作者：
Yabin Gao;Huahua Huang;Shouwen Chen;G. Qi
中科院分区：
文献类型：
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作者：
Yabin Gao;Huahua Huang;Shouwen Chen;G. Qi
2，3-丁二醇（2，3-BD）可以通过发酵芒草等天然资源来生产。地衣芽孢杆菌突变株WX-02Δ budC和WX-02ΔgldA具有利用米曲霉生产光学纯2，3-BD的潜力。WX-02Δ budC和WX-02Δ gldA均能有效利用木糖以及葡萄糖和木糖的混合糖生产光学纯的2，3-BD。M.在摇瓶发酵条件下，WX-02Δ budC和WX-02ΔgldA的产率分别为21.6 g/Ld-2，3-BD和23.9 g/Lmeso-2，3-BD，在生物反应器中分别为13.8 g/Ld-2，3-BD和13.2 g/Lmeso-2，3-BD。进一步在生物反应器中进行流加发酵，结果表明，两株菌均能产生光学纯的2，3-BD，WX-02Δ budC的2，3-BD产量为32.2 g/L，WX-02ΔgldA的2，3-BD产量为48.5 g/L。WX-02Δ budC和WX-02Δ gldA能有效地合成光学纯的2，3-BD。floridulus水解液中的2，3-BD，这两株菌是利用M. floridulushydrolysis.
2,3-Butanediol (2,3-BD) can be produced by fermentation of natural resources likeMiscanthus. Bacillus licheniformismutants, WX-02ΔbudCand WX-02ΔgldA, were elucidated for the potential to useMiscanthusas a cost-effective biomass to produce optically pure 2,3-BD. Both WX-02ΔbudCand WX-02ΔgldAcould efficiently use xylose as well as mixed sugars of glucose and xylose to produce optically pure 2,3-BD. Batch fermentation ofM. floridulushydrolysate could produce 21.6 g/Ld-2,3-BD and 23.9 g/Lmeso-2,3-BD in flask, and 13.8 g/Ld-2,3-BD and 13.2 g/Lmeso-2,3-BD in bioreactor for WX-02ΔbudCand WX-02ΔgldA, respectively. Further fed-batch fermentation of hydrolysate in bioreactor showed both of two strains could produce optically pure 2,3-BD, with 32.2 g/Ld-2,3-BD for WX-02ΔbudCand 48.5 g/Lmeso-2,3-BD for WX-02ΔgldA, respectively. Collectively, WX-02ΔbudCand WX-02ΔgldAcan efficiently produce optically pure 2,3-BD withM. floridulushydrolysate, and these two strains are candidates for industrial production of optical purity of 2,3-BD withM. floridulushydrolysate.