Direct hypothalamic innervation of the trigeminal motor nucleus:: A retrograde tracer study
Direct hypothalamic innervation of the trigeminal motor nucleus:: A retrograde tracer study
复制标题
DOI：
10.1016/j.neuroscience.2005.08.028
复制
发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Morales, FR
中科院分区：
文献类型：
--
作者：
McGregor, R;Damián, A;Morales, FR
目前认为下丘脑通过与前运动结构的连接影响运动输出，而前运动结构又投射到运动核。然而，最近已经证明了对不同运动池的低促肾上腺素能/食欲素能投射。本研究旨在检验豚鼠下丘脑/食欲素能神经元是否是从下丘脑到三叉神经运动核投射的唯一来源。将霍乱毒素 b 亚基注射到三叉神经运动核中，以逆行标记前运动神经元。在下丘脑中观察到两个解剖学上分离的标记神经元群：一组沿着下丘脑外侧区的背侧区、下丘脑背内侧核的外侧部分和穹窿周核分布；另一组沿着下丘脑外侧区的背侧区、下丘脑背内侧核的外侧部分和穹窿周核分布。另一个位于下丘脑背内侧核的室周部分内。两个群体中的许多霍乱毒素亚基 b(+) 神经元都表现出谷氨酸样免疫反应性。此外，含有下丘脑分泌素/食欲素的运动前神经元分布于下丘脑外侧背内侧核、穹窿周核和下丘脑外侧区。对其他前运动神经元进行黑色素浓缩激素免疫染色；这些细胞位于下丘脑外侧区和穹窿周核内，与谷氨酸能和下丘脑/食欲素能神经元混合在一起。氮能前运动神经元仅位于下丘脑背内侧核的室周区。下丘脑运动前神经元均不具有 GABA 能、胆碱能或单胺能。从下丘脑投射到三叉神经运动池的多种神经递质系统的存在表明，这种间脑结构可能会影响三叉神经运动系统所支持的众多功能。 (c) 2005 年由 Elsevier Ltd 代表 IBRO 出版。
It is currently thought that the hypothalamus influences motor output through connections with premotor structures which in turn project to motor nuclei. However, hypocretinergic/orexinergic projections to different motor pools have recently been demonstrated. The present study was undertaken to examine whether hypocretinergic/orexinergic neurons are the only source of projections from the hypothalamus to the trigeminal motor nucleus in the guinea-pig. Cholera toxin subunit b was injected into the trigeminal motor nucleus in order to retrogradely label premotor neurons. Two anatomically separated populations of labeled neurons were observed in the hypothalamus: one group was distributed along the dorsal zone of the lateral hypothalamic area, the lateral portion of the dorsomedial hypothalamic nucleus and the perifornical nucleus; the other was located within the periventricular portion of the dorsomedial hypothalamic nucleus. Numerous cholera toxin subunit b(+) neurons in both populations displayed glutamate-like immunoreactivity. In addition, premotor neurons containing hypocretin/orexin were distributed throughout the lateral dorsomedial hypothalamic nucleus, perifornical nucleus and lateral hypothalamic area. Other premotor neurons were immunostained for melanin concentrating hormone; these cells, which were located within the lateral hypothalamic area and the perifornical nucleus, were intermingled with glutamatergic and hypocretinergic/orexinergic neurons. Nitrergic premotor neurons were located only in the periventricular zone of the dorsomedial hypothalamic nucleus. None of the hypothalamic premotor neurons were GABAergic, cholinergic or monoaminergic.The existence of diverse neurotransmitter systems projecting from the hypothalamus to the trigeminal motor pool indicates that this diencephalic structure may influence the numerous functions that are subserved by the trigerninal motor system. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.