Isolation and analysis of recombinant DNA molecules containing yeast DNA.
Isolation and analysis of recombinant DNA molecules containing yeast DNA.
复制标题
含有酵母 DNA 的重组 DNA 分子的分离和分析。
DOI：
10.1016/0378-1119(78)90013-6
复制
发表时间：
1978
期刊：
影响因子：
3.5
通讯作者：
D. Botstein
中科院分区：
文献类型：
--
作者：
T. Petes;T. Petes;J. Broach;P. C. Wensink;L. Hereford;G. Fink;D. Botstein
已在大肠杆菌中克隆了2500个含有酵母核DNA插入片段的重组质粒。可以计算出，约85%的酵母基因组在该集合中被代表。已通过与纯化的RNA种类杂交来表征克隆。在2000个被检测的克隆中，75个含有酵母核糖体DNA的插入，201个含有酵母tRNA基因的插入，26个含有与丰富的mRNA种类互补的DNA序列。
2500 recombinant plasmids containing insertions of yeast nuclear DNA have been cloned inEscherichia coli. It can be calculated that about 85% of the yeast genome is represented in this collection. The clones have been characterized by hybridization to purified RNA species. Of the 2000 clones examined, 75 contain insertions of yeast ribosomal DNA, 201 contain insertions of yeast tRNA genes, and 26 contain DNA sequences that are complementary to abundant mRNA species.