Detection of elastin in the human fetal membranes: Proposed molecular basis for elasticity
Detection of elastin in the human fetal membranes: Proposed molecular basis for elasticity
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DOI：
10.1016/s0143-4004(97)80065-3
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发表时间：
1997-05-01
期刊：
影响因子：
3.8
通讯作者：
BryantGreenwood, GD
中科院分区：
文献类型：
--
作者：
Hieber, AD;Corcino, D;BryantGreenwood, GD
人的胎膜提供了一个无菌的生物力学容器，通过生长调节到妊娠中期，以适应胎儿尺寸的增加，并通过弹性调节到胎儿的有力运动。这种弹性的分子基础尚不清楚，但弹性降低可能导致其过早破裂和早产，这是围产期医学的主要问题。传统上，弹性蛋白赋予弹性纤维弹性回缩的性质，所述弹性纤维由原弹性蛋白前体家族组装而成。它们通过赖氨酰氧化酶催化形成锁链素和异锁链素而共价交联形成不溶性弹性蛋白。通过北方分析和RT-PCR显示羊膜、绒毛膜和蜕膜含有可检测水平的原弹性蛋白mRNA和编码赖氨酰氧化酶的mRNA。这些mRNA编码的蛋白质也通过Western印迹和免疫定位进行了鉴定。此外，从人胎膜中提取不溶性弹性蛋白，并通过与来自其他弹性组织的弹性蛋白制剂进行比较，显示其具有合理的锁链素含量。最后，扫描电子显微镜证实，在这个组织中存在多层明显非常薄的弹性系统。因此，这项生物化学和组织病理学研究表明，人胎膜合成并存款一种新的弹性纤维。这种弹性系统在这些组织中的存在首次为这种组织的弹性性质提供了可能的分子基础。(C)小行星1997 W. B。Saunders Company Ltd
The human fetal membranes provide a sterile biomechanical container which adjust by growth to mid-pregnancy to the increase in fetal size, and by elasticity to the forceful movements of the fetus. The molecular basis for this elasticity is not known, yet reduced elasticity may lead to their premature rupture and preterm birth, a major problem in perinatal medicine. Classically, elastin confers the property of elastic recoil to elastic fibres which are assembled from a family of tropoelastin precursors. These are covalently cross-linked to form insoluble elastin by formation of desmosine and isodesmosine, catalysed by the enzyme lysyl oxidase. The amnion, chorion and decidua were shown by Northern analysis and RT-PCR to contain detectable levels of tropoelastin mRNA and the mRNA encoding lysyl oxidase. The proteins encoded by these mRNAs were also identified by Western blotting and immunolocalization. Further, insoluble elastin was extracted from the human fetal membranes and shown by comparison to elastin preparations from other elastic tissues to have a reasonable desmosine content. Finally, scanning electron microscopy confirmed the presence of multiple layers of an apparently very thin elastic system in this tissue. This biochemical and histopathologic study has demonstrated therefore that the human fetal membranes synthesize and deposit a novel elastic fibre. The presence of such an elastic system in these tissues provides, for the first time, a probable molecular basis for the elastic properties of this tissue. (C) 1997 W. B. Saunders Company Ltd.