Patients with IgA nephropathy have circulating anti-basement membrane antibodies reacting with structures common to collagen I, II, and IV.
Patients with IgA nephropathy have circulating anti-basement membrane antibodies reacting with structures common to collagen I, II, and IV.
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IgA 肾病患者体内存在循环抗基底膜抗体，与 I、II 和 IV 型胶原蛋白的常见结构发生反应。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
D. Heinegård
中科院分区：
文献类型：
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作者：
B. Cederholm;J. Wieslander;P. Bygren;D. Heinegård
最近已经表明，伊加肾病患者具有针对人肾小球基底膜提取物的循环伊加抗体。本研究扩展了这些观察结果，并通过ELISA和免疫印迹技术证明伊加肾病患者循环中存在针对IV型胶原α链的伊加抗体。α链的抗原性可通过用胶原酶消化而被破坏，这表明抗原位点位于胶原IV的三螺旋部分上。此外，通过抑制试验表明，伊加抗体针对也存在于胃蛋白酶消化后分离的胶原I和II中的表位。
It has recently been shown that patients with IgA nephropathy have circulating IgA antibodies against extracts of human glomerular basement membrane. The present study extends those observations and demonstrates by using ELISA and immunoblotting techniques that patients with IgA nephropathy have circulating IgA antibodies against collagen IV alpha chains. The antigenicity of the alpha chains could be destroyed by digestion with collagenase, which indicates that the antigenic site(s) is located on the triple helical part of collagen IV. Furthermore, it was shown by inhibition tests that the IgA antibodies are directed against epitopes also present in collagen I and II isolated after pepsin digestion.