Molecular characterization of canine kobuvirus in wild carnivores and the domestic dog in Africa
Molecular characterization of canine kobuvirus in wild carnivores and the domestic dog in Africa
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DOI：
10.1016/j.virol.2015.01.010
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发表时间：
2015-03-01
期刊：
影响因子：
3.7
通讯作者：
East, Marion L.
中科院分区：
文献类型：
--
作者：
Olarte-Castillo, Ximena A.;Heeger, Felix;East, Marion L.
关于肉食性动物感染柯布病毒的知识有限，还没有在非洲家养或野生肉食性动物中描述过。为了填补这一知识空白，我们使用RT-PCR对几种非洲食肉动物的新鲜粪便进行了筛选。我们从家犬、金丝狼、横纹胡狼和斑点鬣狗的样本中检测到柯布病毒RNA。使用下一代测序，我们从每个物种中获得了一个完整的KobuVirus基因组序列。我们的系统发育分析揭示了犬柯布病毒(CaKV)在所有四个物种中的感染，并将来自非洲的CaKV放在一起，并与来自其他地方的CaKV分开。野生食肉动物菌株之间的亲缘关系比与家犬的亲缘关系更近。我们发现IRES的二级结构模型类似于AichiVirus-like IRES亚类，并且在非洲毒株中是保守的。我们描述了来自非洲的第一批CaKV，并扩大了已知的CaKV宿主范围。(C)2015 Elsevier Inc.保留所有权利。
Knowledge of Kobuvirus (Family Picornaviridae) infection in carnivores is limited and has not been described in domestic or wild carnivores in Africa. To fill this gap in knowledge we used RT-PCR to screen fresh feces from several African carnivores. We detected kobuvirus RNA in samples from domestic dog, golden jackal, side-striped jackal and spotted hyena. Using next generation sequencing we obtained one complete Kobuvirus genome sequence from each of these species. Our phylogenetic analyses revealed canine kobuvirus (CaKV) infection in all four species and placed CaKVs from Africa together and separately from CaKVs from elsewhere. Wild carnivore strains were more closely related to each other than to those from domestic dogs. We found that the secondary structure model of the IRES was similar to the Aichivirus-like IRES subclass and was conserved among African strains. We describe the first CaKVs from Africa and extend the known host range of CaKV. (C) 2015 Elsevier Inc. All rights reserved.