Effects of tensile and compressive strains on response of a chondrocytic cell line embedded in type I collagen gel
Effects of tensile and compressive strains on response of a chondrocytic cell line embedded in type I collagen gel
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DOI：
10.1016/j.jbiotec.2007.07.955
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发表时间：
2008-01-20
影响因子：
4.1
通讯作者：
Naruse, Keiji
中科院分区：
文献类型：
--
作者：
Hirano, Yuji;Ishiguro, Naoki;Naruse, Keiji
拉伸和压缩应变通常用于机械生物学模型。在这里，我们报告一种新的三维细胞培养方法的发展，它允许拉伸和压缩载荷施加。使用包埋在I型胶原凝胶中的HCS 2/8软骨细胞获得初步结果。该结构承受16%拉伸或14%压缩。共聚焦激光扫描显微镜显示，拉伸和压缩引起显着的细胞变形。将胶原凝胶包埋的HCS 2/8细胞进行静态拉伸、动态拉伸、静态压缩或动态压缩24 h。与对照组相比，动态压缩导致5-溴-2 '-脱氧尿苷掺入显著降低。PCR分析显示，动态张力引起的II型胶原蛋白的上调，静态压缩引起的聚集蛋白聚糖的上调，以及动态压缩引起的II型胶原蛋白和聚集蛋白聚糖的下调。与对照组相比，静态拉伸和静态压缩显著增加了一氧化氮的产生。我们的实验系统有效地将几种类型的菌株应用于包埋在胶原凝胶中的HCS 2/8细胞。我们的研究结果表明，机械应变的方式影响的HCS 2/8细胞的反应。(c)2007 Elsevier B. V.保留所有权利。
Tensile and compressive strains are commonly used in mechanobiological models. Here we report on the development of a novel three-dimensional cell-culture method, which allows both tensile and compressive loads to be applied. Preliminary results were obtained using HCS2/8 chondrocytic cells embedded in type I collagen gel. This construct was subjected to either 16% tension or 14% compression. Confocal laser scanning microscopy showed that both tension and compression caused significant cell deformation. The collagen gel-embedded HCS2/8 cells were subjected to static tension, dynamic tension, static compression or dynamic compression for 24 h. Dynamic compression led to significantly decreased 5-bromo-2'-deoxyuridine incorporation compared with the control group. PCR analysis revealed upregulation of type II collagen caused by dynamic tension, upregulation of aggrecan caused by static compression, and downregulation of type II collagen and aggrecan caused by dynamic compression. Nitric oxide production was significantly increased by static tension and static compression compared with the control group. Our experimental system effectively applied several types of strain to HCS2/8 cells embedded in collagen gel. Our results suggest that the mode of mechanical strain affects the response of HCS2/8 cells. (c) 2007 Elsevier B.V. All rights reserved.