Catechin hydrate inhibits proliferation and mediates apoptosis of SiHa human cervical cancer cells
Catechin hydrate inhibits proliferation and mediates apoptosis of SiHa human cervical cancer cells
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DOI：
10.1016/j.fct.2011.09.023
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发表时间：
2011-12-01
影响因子：
4.3
通讯作者：
Alshatwi, Ali A.
中科院分区：
文献类型：
--
作者：
Al-Hazzani, Amal A.;Alshatwi, Ali A.
儿茶素水合物（CH）是绿色茶中的一种化合物，已被证明可以抑制肿瘤生长。绿色茶具有抗癌潜力，是世界上最常用的草药之一。在这项研究中，我们试图表征的DNA损伤和下游基因的CH提取物使用SiHa人宫颈癌细胞的目标。本研究旨在探讨CH在体外对宫颈癌细胞的杀伤作用，以确定CH是否具有抗癌潜力，并在进一步研究后可开发为宫颈癌的治疗剂。为了科学地验证CH对宫颈癌的抗癌活性，测试了CH的细胞毒性和生长抑制特性，特别是SiHa宫颈癌细胞凋亡的诱导。CH在196.07 μ g/mL浓度下24 h对SiHa人宫颈癌细胞的抑制率为50%。CH在24 h和48 h诱导caspase-3、-8和-9基因表达成倍增加，这些基因表达的增加可能与诱导细胞凋亡有关。通过DeadEnd末端转移酶介导的dUTP-digoxigenin-end labeling（TUNEL）分析细胞凋亡以进一步证实CH诱导细胞凋亡。结果表明，CH有可能有利于宫颈癌的预防。这是第一份报告，显示了可能的机制，抗增殖作用的CH在预防宫颈癌的细胞培养模型。CH，无论是以其原始形式还是与其他抗癌药物组合，都可能成为宫颈癌的替代药物。进一步的研究可能会增加我们对CH在宫颈癌治疗中的作用机制的理解。(C)2011爱思唯尔有限公司保留所有权利。
Catechin hydrate (CH), one of the chemical compounds in green tea, has been shown to inhibit tumor growth. Green tea possesses anticancer potential and is one of the most commonly used herbal medicines worldwide. In this study, we sought to characterize the DNA damage and downstream genes targeted by CH extracts using SiHa human cervical cancer cells. The efficacy of CH in killing cervical cancer cells in vitro was investigated in this study to determine whether CH possesses anticancer potential and could be developed as a therapeutic agent for cervical cancer upon further investigation. To scientifically validate the anticancer activities of CH on cervical cancer, CH was tested for its cytotoxic and growth-inhibition properties, specifically the induction of apoptosis in SiHa cervical cancer cells. CH showed a 50% inhibition of SiHa human cervical cancer cells at a concentration of 196.07 mu g/mL at 24 h. CH induced the several folds increase of caspase-3, -8, and -9 after 24 h and 48 h; the increase of these genes may be involved in the induction of apoptosis. The analysis of apoptosis by DeadEnd terminal transferase-mediated dUTP-digoxigenin-end labeling (TUNEL) assay was used to further confirm that CH induced apoptosis. The results suggested that CH has the potential to benefit cervical cancer prevention. This is the first report that shows the possible mechanism of the anti-proliferative effects of CH in the prevention of cervical cancer in cell culture models. CH, either in its original form or in combination with other anticancer drugs, could potentially be an alternative medicine for cervical cancer. Further study may increase our understanding of the mechanism by which CH has an effect on cervical cancer therapy. (C) 2011 Elsevier Ltd. All rights reserved.