Dibutyl phthalate induces oxidative stress and impairs spermatogenesis in adult rats
Dibutyl phthalate induces oxidative stress and impairs spermatogenesis in adult rats
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DOI：
10.1177/0748233714566877
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发表时间：
2016-08-01
影响因子：
1.9
通讯作者：
Osman, Abdel-Moneim M.
中科院分区：
文献类型：
--
作者：
Aly, Hamdy A. A.;Hassan, Memy H.;Osman, Abdel-Moneim M.
邻苯二甲酸酯是产量丰富的增塑剂，邻苯二甲酸二丁酯（DBP）是各种消费品和医疗器械中使用最广泛的衍生物。本研究旨在进一步探讨 DBP 对成年大鼠的潜在睾丸毒性并阐明其潜在机制。成年雄性白化大鼠连续 15 天口服 DBP，剂量为 0、200、400 或 600 mg/kg/天。睾丸重量、精子数量和活力显着下降。 DBP治疗降低了血清促卵泡激素和睾酮水平以及睾丸乳酸脱氢酶活性。 DBP治疗还降低了血清总抗氧化能力和睾丸抗氧化酶的活性，例如超氧化物歧化酶、过氧化氢酶和谷胱甘肽还原酶。此外，DBP 治疗还会引起一些生精小管的退化，导致生精和精子丧失以及坏死。这些结果表明氧化应激和随后的睾酮分泌减少是 DBP 诱导睾丸毒性的潜在潜在机制。
Phthalates are abundantly produced plasticizers, and dibutyl phthalate (DBP) is the most widely used derivative in various consumer products and medical devices. This study was conducted to further explore the potential testicular toxicity of DBP in adult rats and to elucidate the underlying mechanisms. Adult male albino rats were treated orally with DBP at doses of 0, 200, 400, or 600 mg/kg/day for 15 consecutive days. Testicular weight, sperm count, and motility were significantly decreased. Treatment with DBP decreased serum follicle-stimulating hormone and testosterone levels and testicular lactate dehydrogenase activity. DBP treatment also decreased serum total antioxidant capacity and the activities of the testicular antioxidant enzymes, such as superoxide dismutase, catalase, and glutathione reductase. Further, DBP treatment provoked degeneration with absence of spermatogenesis and sperms and necrosis in some of seminiferous tubules. These results indicated that oxidative stress and subsequent decrease in testosterone secretion were the potential underlying mechanism of DBP-induced testicular toxicity.