Characterization and Cloning of Grape Circular RNAs Identified the Cold Resistance-Related Vv-circATS1
Characterization and Cloning of Grape Circular RNAs Identified the Cold Resistance-Related Vv-circATS1
复制标题
葡萄环状RNA的表征和克隆鉴定出抗寒相关的Vv-circATS1
DOI：
10.1104/pp.18.01331
复制
发表时间：
2019-06-01
期刊：
影响因子：
7.4
通讯作者：
Ma, Chao
中科院分区：
文献类型：
--
作者：
Gao, Zhen;Li, Jing;Ma, Chao
环状 RNA (circRNA) 分布广泛，在一系列发育过程中发挥重要作用，但尚未在葡萄树 (Vitis vinifera) 中鉴定或表征。在这项研究中，我们表征了葡萄 circRNA 的功能，并通过全局转录组分析发现了数千个假定的反向剪接位点。我们的结果表明，应同时使用几种已报道的 circRNA 预测算法，以获得植物中全面且可靠的 circRNA 预测。此外，葡萄circRNA侧翼的内含子长度与外显子环化密切相关。尽管葡萄 circRNA 侧翼较长的内含子似乎环化效率更高，但 20 至 50 nt 的区域似乎足以驱动葡萄 circRNA 的生物发生。此外，环化外显子侧翼的内源内含子与反向互补序列相结合，可以支持葡萄中各种外显子的准确有效的环化，这构成了探索circRNA表达引起的功能后果的新工具。最后，我们鉴定了冷胁迫下葡萄叶片中 475 个差异表达的 circRNA。 Vv-circATS1（一种源自甘油-3-P酰基转移酶的环状RNA）的过表达可提高拟南芥（Arabidopsis thaliana）的耐寒性，而源自相同序列的线性RNA却不能。这些结果表明了circRNA和线性RNA之间的功能差异，并为植物非生物胁迫抗性提供了新的见解。
Circular RNAs (circRNAs) are widely distributed and play essential roles in a series of developmental processes, although none have been identified or characterized in grapevines (Vitis vinifera). In this study, we characterized the function of grape circRNA and uncovered thousands of putative back-splicing sites by global transcriptome analysis. Our results indicated that several reported circRNA prediction algorithms should be used simultaneously to obtain comprehensive and reliable circRNA predictions in plants. Furthermore, the length of introns flanking grape circRNAs was closely related to exon circularization. Although the longer introns flanking grape circRNAs appeared to circularize more efficiently, a 20- to 50-nt region seemed large enough to drive grape circRNA biogenesis. In addition, the endogenous introns flanking circularized exon(s) in conjunction with reverse complementary sequences could support the accurate and efficient circularization of various exons in grape, which constitutes a new tool for exploring the functional consequences caused by circRNA expression. Finally, we identified 475 differentially expressed circRNAs in grape leaves under cold stress. Overexpression of Vv-circATS1, a circRNA derived from glycerol-3-P acyltransferase, improved cold tolerance in Arabidopsis (Arabidopsis thaliana), while the linear RNA derived from the same sequence cannot. These results indicate the functional difference between circRNA and linear RNA, and provide new insight into plant abiotic stress resistance.