DNA-DIRECTED AND RNA-PRIMED DNA-SYNTHESIS IN MICROSOMAL AND MITOCHONDRIAL FRACTIONS OF NORMAL HUMAN LYMPHOCYTES
DNA-DIRECTED AND RNA-PRIMED DNA-SYNTHESIS IN MICROSOMAL AND MITOCHONDRIAL FRACTIONS OF NORMAL HUMAN LYMPHOCYTES
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DOI：
10.1016/0006-291x(74)90635-4
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发表时间：
1974-01-01
影响因子：
3.1
通讯作者：
GALLO, RC
中科院分区：
文献类型：
--
作者：
REITZ, MS;SMITH, RG;GALLO, RC
由植物血凝素刺激的正常人血淋巴细胞的微粒体和线粒体部分的 RNase 敏感内源 DNA 聚合酶活性合成的新生 DNA 共价连接到小 RNA 分子（微粒体部分<4S）。该复合物最初又与一个更大的先前存在的 DNA 分子氢键结合，表明 DNA 的合成是由 DNA（模板）指导的，而 RNA 仅用作起始分子（引物）。这种内源性 RNase 敏感 DNA 合成不应与 C 型 RNA 肿瘤病毒和人白血病细胞中由逆转录酶催化的内源 RNase 敏感 DNA 合成相混淆，逆转录酶利用 RNA 作为模板和引物。这些发现与哺乳动物细胞器中的某些DNA合成可能类似于细菌利用带有DNA模板的RNA引物的概念是一致的。
Nascent DNA synthesized from RNase-sensitive endogenous DNA polymerase activities of microsomal and mitochondrial fractions from phytohemagglutinin-stimulated normal human blood lymphocytes is covalently attached to a small RNA molecule (<4S in the case of the microsomal fraction). This complex is in turn originally hydrogen bonded to a much larger preexistent DNA molecule, indicating that this synthesis of DNA is directed by DNA (template) and that the RNA is utilized solely as an initiator molecule (primer). This endogenous RNase-sensitive DNA synthesis should not be confused with the endogenous RNase-sensitive DNA synthesis in type-C RNA tumor viruses and in related cytoplasmic particles from human leukemic cells catalyzed by reverse transcriptase which utilizes RNA as both template and primer. These findings are consistent with the concept that some DNA synthesis in mammalian cell organelles may be analogous to that of bacteria in utilizing RNA primers with DNA templates.