Co-immobilization of Biomolecules on Ultrathin Reactive Chemical Vapor Deposition Coatings Using Multiple Click Chemistry Strategies
Co-immobilization of Biomolecules on Ultrathin Reactive Chemical Vapor Deposition Coatings Using Multiple Click Chemistry Strategies
复制标题
DOI：
10.1021/am401875x
复制
发表时间：
2013-10-09
影响因子：
9.5
通讯作者：
Lahann, Joerg
中科院分区：
文献类型：
--
作者：
Bally, Florence;Cheng, Kenneth;Lahann, Joerg
生物分子（如蛋白质或糖）的固定化是生物技术中的一个关键问题，因为它使人们能够了解更多生物相关环境中的细胞行为。在这里，聚（4-乙炔基-对-亚二甲苯-共-对-亚二甲苯）涂层已经通过化学气相沉积（CVD）聚合来制造，以便将生物活性分子结合到材料的表面上。通过调节用于沉积的前体的量来实现对CVD膜的厚度的控制。然后通过微接触印刷进行铜催化的Huisgen环加成，以将各种生物分子固定在反应性涂层上。这种点击化学反应的选择性已经通过荧光糖识别分子（凝集素）的空间控制缀合以及细胞粘附到肽图案上来证实。此外，可以经历多个点击化学反应的微结构化涂层已经通过两个连续的CVD步骤开发。聚（4-乙炔基-对-二甲苯-co-对-二甲苯）和聚（4-甲酰基-对-二甲苯-co-对-二甲苯）已经通过复制结构中的气相辅助微图案化（VAMPIR）进行图案化。Huisgen环加成和羰基-酰肼偶联的组合用于在空间上引导糖在图案化基底上的固定。这项工作开辟了新的视角，在剪裁微结构，多反应界面，可以通过生物正交化学装饰作为模仿细胞的生物环境。
Immobilization of biomolecules, such as proteins or sugars, is a key issue in biotechnology because it enables the understanding of cellular behavior in more biological relevant environment. Here, poly(4-ethynyl-p-xylylene-co-p-xylylene) coatings have been fabricated by chemical vapor deposition (CVD) polymerization in order to bind bioactive molecules onto the surface of the material. The control of the thickness of the CVD films has been achieved by tuning the amount of precursor used for deposition. Copper-catalyzed Huisgen cycloaddition has then been performed via microcontact printing to immobilize various biomolecules on the reactive coatings. The selectivity of this click chemistry reaction has been confirmed by spatially controlled conjugation of fluorescent sugar recognizing molecules (lectins) as well as cell adhesion onto the peptide pattern. In addition, a microstructured coating that may undergo multiple click chemistry reactions has been developed by two sequential CVD steps. Poly(4-ethynyl-p-xylylene-co-p-xylylene) and poly(4-formyl-p-xylylene-co-p-xylylene) have been patterned via vapor-assisted micropatterning in replica structures (VAMPIR). A combination of Huisgen cycloaddition and carbonyl-hydrazide coupling was used to spatially direct the immobilization of sugars on a patterned substrate. This work opens new perspectives in tailoring microstructured, multireactive interfaces that can be decorated via bio-orthogonal chemistry for use as mimicking the biological environment of cells.