LINKER HISTONES ARE NOT ESSENTIAL AND AFFECT CHROMATIN CONDENSATION IN-VIVO
LINKER HISTONES ARE NOT ESSENTIAL AND AFFECT CHROMATIN CONDENSATION IN-VIVO
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DOI：
10.1016/0092-8674(95)90051-9
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发表时间：
1995-07-14
期刊：
影响因子：
64.5
通讯作者：
GOROVSKY, MA
中科院分区：
文献类型：
--
作者：
SHEN, XT;YU, LL;GOROVSKY, MA
我们已经（分别）破坏了所有的表达的大核拷贝的HHO基因编码的大核组蛋白H1和微核接头组蛋白（MLH）基因编码的蛋白质MicLH在四膜虫嗜热。这些破坏显示完全消除每种蛋白质的表达。没有连接组蛋白的菌株以正常速率生长并达到接近正常的细胞密度，表明连接组蛋白对于细胞存活不是必需的。组蛋白H1敲除（Delta H1）细胞具有扩大的DAPI染色大核和正常大小的微核，而MicLH敲除（Delta MicLH）细胞具有扩大的微核和正常大小的大核。Δ MicLH细胞正常进行有丝分裂。然而，微核有丝分裂染色体结构是不太凝聚。这些研究提供的证据表明，接头组蛋白是非必需的，并参与染色质包装和凝聚在体内。
We have (separately) disrupted all of the expressed macronuclear copies of the HHO gene encoding macronuclear histone H1 and of the micronuclear linker histone (MLH) gene encoding the protein MicLH in Tetrahymena thermophila. These disruptions are shown to eliminate completely the expression of each protein. Strains without either linker histone grow at normal rates and reach near-normal cell densities, demonstrating that linker histones are not essential for cell survival. Histone H1 knockout (Delta H1) cells have enlarged DAPI-stained macronuclei and normal-sized micronuclei, while MicLH knockout (Delta MicLH) cells have enlarged micronuclei and normal-sized macronuclei. Delta MicLH cells undergo mitosis normally. However, the micronuclear mitotic chromosome structure is less condensed. These studies provide evidence that linker histones are nonessential and are involved in chromatin packaging and condensation in vivo.