Immunization with non-replicating E-coli minicells delivering both protein antigen and DNA protects mice from lethal challenge with lymphocytic choriomeningitis virus
Immunization with non-replicating E-coli minicells delivering both protein antigen and DNA protects mice from lethal challenge with lymphocytic choriomeningitis virus
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DOI：
10.1016/j.vaccine.2006.11.069
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发表时间：
2007-03-08
期刊：
影响因子：
5.5
通讯作者：
McGuire, Kathleen L.
中科院分区：
文献类型：
--
作者：
Giacalone, Matthew J.;Zapata, Juan C.;McGuire, Kathleen L.
目前正在对新疫苗和疫苗载体的输送和保护机制进行新的调查。很明显，不涉及活的、可复制的生物体的免疫传递机制更受欢迎。在本报告中，研究人员测试了非复制性细菌小细胞同时共同递送淋巴细胞性脉络丛脑膜炎病毒（LCMV）核蛋白（NP）和相应的DNA疫苗在小鼠体内产生保护性细胞免疫反应的能力。结果发现，肌内注射获得了良好的保护（89%），鼻内注射获得了中等程度的保护（31%），胃免疫获得了较低的保护（7%）。这些结果为利用细菌微细胞作为非复制性疫苗递送平台提供了坚实的基础。(c) 2006 Elsevier Ltd.版权所有。
In the midst of new investigations into the mechanisms of both delivery and protection of new vaccines and vaccine carriers. it has become clear that immunization with delivery mechanisms that do not involve living, replicating organisms are vastly preferred. In this report, nonreplicating bacterial minicells simultaneously co-delivering the nucleoprotein (NP) of lymphocytic choriomeningitis virus (LCMV) and the corresponding DNA vaccine were tested for the ability to generate protective cellular immune responses in mice. It was found that good protection (89%) was achieved after intramuscular administration, moderate protection (31%) was achieved after intranasal administration, and less protection (7%) was achieved following gastric immunization. These results provide a solid foundation on which to pursue the use of bacterial minicells as a non-replicating vaccine delivery platform. (c) 2006 Elsevier Ltd. All rights reserved.