Nuclear envelope dynamics during plant cell division suggest common mechanisms between kingdoms
Nuclear envelope dynamics during plant cell division suggest common mechanisms between kingdoms
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DOI：
10.1042/bj20101769
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发表时间：
2011-05-01
影响因子：
4.1
通讯作者：
Evans, David E.
中科院分区：
文献类型：
--
作者：
Graumann, Katia;Evans, David E.
在后生动物开放有丝分裂过程中NE（核膜）的行为已被广泛研究，但缺乏天然标记限制了植物中类似的研究。在本研究中，进行使用活同步烟草BY-2悬浮培养，非功能性NE标记LBR（核纤层蛋白B受体）GFP（绿色荧光蛋白）和两个本地的，功能性NE蛋白，AtSUN 1 [拟南芥SUN（Sad 1/UNC 84）1]和AtSUN 2，我们提供的证据表明，ER（内质网）保留理论NE膜是适用于植物。我们还观察到两个明显独特的植物功能：位置的NE-膜组件在整个分裂过程中接近染色质，和空间上不同的改造NE开始在染色质表面面向纺锤体两极，并在面对细胞板的表面结束。研究了NE间期、NE分解和重组期间、纺锤体膜和细胞板中蛋白质的迁移率。AtSUN 2在核膜破裂中的作用。
Behaviour of the NE (nuclear envelope) during open mitosis has been explored extensively in metazoans, but lack of native markers has limited similar investigations in plants. In the present study, carried out using living synchronized tobacco BY-2 suspension cultures, the non-functional NE marker LBR (lamin B receptor) GFP (green fluorescent protein) and two native, functional NE proteins, AtSUN1 [Arapidopsis thaliana SUN (Sad1/UNC84) 1] and AtSUN2, we provide evidence that the ER (endoplasmic reticulum)-retention theory for NE membranes is applicable in plants. We also observe two apparently unique plant features: location of the NE-membrane components in close proximity to chromatin throughout division, and spatially distinct reformation of the NE commencing at the chromatin surface facing the spindle poles and concluding at the surface facing the cell plate. Mobility of the proteins was investigated in the interphase NE, during NE breakdown and reformation, in the spindle membranes and the cell plate. A role for AtSUN2 in nuclear envelope breakdown is suggested.