CYTOPLASMIC AND NUCLEAR VIRUS-SPECIFIC PROTEINS IN INFLUENZA VIRUS-INFECTED MDCK CELLS
CYTOPLASMIC AND NUCLEAR VIRUS-SPECIFIC PROTEINS IN INFLUENZA VIRUS-INFECTED MDCK CELLS
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DOI：
10.1016/0042-6822(73)90310-3
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发表时间：
1973-01-01
期刊：
影响因子：
3.7
通讯作者：
ETKIND, PR
中科院分区：
文献类型：
--
作者：
KRUG, RM;ETKIND, PR
从流感病毒感染细胞中纯化的细胞核中鉴定出4种病毒特异性蛋白：核衣壳蛋白（NP）；两个分子量为24,500道尔顿（NS1）和11,000道尔顿（NS2）的非结构蛋白；发现核衣壳蛋白在核质和核仁部分之间的分布大致相等，而其他大多数病毒特异性蛋白与核仁部分相关。在核质中，大多数（如果不是全部的话）核衣壳蛋白是以病毒核糖核蛋白的形式存在的。除了存在于细胞核中，ns1还被发现集中在含有多核糖体和核糖体的细胞质中（Pons, 1972; Compans, 1973）。这表明ns1可能作为一种结构核糖体蛋白与核糖体结合，因此，ns1在核仁中是为了被整合到新组装的核糖体亚基中。我们的研究表明，在感染细胞的细胞质提取物中，ns1实际上与核糖体相关，但不是作为结构蛋白；它通过盐解离键简单地吸附在大核糖体亚基和小核糖体亚基上。因此，核仁中的ns1不能作为核糖体结构蛋白的前体。
Four virus-specific proteins were identified in nuclei purified from influenza virusinfected cells: the nucleocapsid protein (NP); two nonstructural proteins of 24,500 daltons (NS1) and 11,000 daltons (NS2) molecular weight; and the largest virion protein, protein P. The nucleocapsid protein was found to be distributed approximately equally between the nucleoplasmic and nucleolar fractions, whereas most of the other virus-specific proteins were associated with the nucleolar fraction. In the nucleoplasm, most, if not all, of the nucleocapsid protein was in the form of viral ribonucleoproteins.In addition to being in the nucleus, NS1has been found concentrated in cytoplasmic fractions containing polyribosomes and ribosomes (Pons, 1972; Compans, 1973). This suggested that NS1might be associated with ribosomes as a structural ribosomal protein and that, therefore, NS1was in the nucleolus for the purpose of being incorporated into newly assembled ribosomal subunits. Our studies show that in cytoplasmic extracts of infected cells, NS1was actually associated with ribosomes, butnotas a structural protein; it was simply adsorbed to both the large and the small ribosomal subunits by salt-dissociable bonds. Consequently, NS1in the nucleolus isnotserving as precursor to a ribosomal structural protein.