5-fluoro-orotic acid induces chromosome alterations in genetically manipulated strains of Candida albicans
5-fluoro-orotic acid induces chromosome alterations in genetically manipulated strains of Candida albicans
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DOI：
10.3852/mycologia.98.3.393
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发表时间：
2006-05-01
期刊：
影响因子：
2.8
通讯作者：
Rustchenko, Elena
中科院分区：
文献类型：
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作者：
Wellington, Melanie;Kabir, M. Anaul;Rustchenko, Elena
我们先前报道了5-氟乳清酸（5-FOA）处理的白色念珠菌原养型菌株3153 A染色体发生改变。在这项研究中，我们研究了5-FOA与两个衍生物的C。白色念珠菌菌株CAF 4 -2（ura 3/ura 3），每个菌株在不同的染色体上含有URA 3基因的异位拷贝（ura 3/ura 3URA 3）。如所预期的，在将ura 3/ura 3URA 3构建体应用于含有5-FOA的固体培养基后，出现了丢失URA 3的“突出物”。然而，大多数“弹出”获得各种染色体改变。因此，应检查暴露于5- 170 A的构建体的染色体改变或应避免使用5 FOA。
We previously reported the occurrence of chromosome alterations in a Candida albicans prototrophic strain 3153A treated with 5-fluoro-orotic acid (5-FOA). In this study we investigated the mutagenic properties of 5-FOA with two derivatives of C. albicans strain CAF4-2 (ura3/ura3), each containing an ectopic copy of URA3 gene (ura3/ ura3 URA3) on a different chromosome. As expected, after the ura3/ura3 URA3 constructs were applied to 5-FOA containing solid medium, the "popouts" that lost URA3 appeared. However most of the ''pop-outs" acquired various chromosome alterations. Thus constructs exposed to 5-170A should be examined for chromosome alterations or the use of 5FOA should be avoided.