Bacterial diversity in persistent periapical lesions on root-filled teeth.
Bacterial diversity in persistent periapical lesions on root-filled teeth.
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DOI：
10.3402/jom.v1i0.1946
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发表时间：
2009-05-21
影响因子：
4.5
通讯作者：
Sunde PT
中科院分区：
文献类型：
--
作者：
Handal T;Caugant DA;Olsen I;Sunde PT
本研究的目的是通过使用不依赖于培养物的分子方法来分析根充牙持续性根尖病变中的细菌多样性。使用针对 16s 核糖体 RNA 基因的 PCR 检测 20 个手术切除的治疗抵抗牙齿根尖病变是否存在细菌 DNA，然后进行克隆和测序。 20 个样本中有 17 个样本检测到细菌 DNA（85%）。总共分析了 236 个克隆。代表了 7 个不同的细菌门，总共鉴定了 75 个不同的细菌类群； 36%的物种尚未被栽培。常见的细菌种类包括梭杆菌属、普氏菌属、连翘坦氏菌、牙髓卟啉单胞菌、齿垢密螺旋体、拟杆菌属、消化链球菌属和链球菌属。在治疗抵抗性牙齿的根尖周病变中发现了多种细菌。这些细菌可能是根尖周感染的病因，并阻碍这些病变的愈合。
The purpose of this study was to analyze the bacterial diversity in persistent apical lesions on root-filled teeth by using culture-independent molecular methods. Twenty surgically removed apical lesions from therapy-resistant teeth were examined for the presence of bacterial DNA using PCR targeting the 16s ribosomal RNA gene, followed by cloning and sequencing. Bacterial DNA was detected in 17 of the 20 samples (85%). A total of 236 clones were analyzed. Seven different bacterial phyla were represented and a total of 75 different bacterial taxa were identified; 36% of the species have not yet been cultivated. Commonly detected bacterial species included Fusobacterium spp., Prevotella spp., Tannerella forsythia, Porphyromonas endodontalis, Treponema denticola, Bacteroidetes spp., Peptostreptococcus spp., and Streptococcus spp. A wide range of bacteria was identified in periapical lesions on therapy-resistant teeth. These bacteria may contribute in the etiology of periapical infection and impede healing of these lesions.