Hyaluronan metabolism in overloaded temporomandibular joint.
Hyaluronan metabolism in overloaded temporomandibular joint.
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DOI：
10.1111/joor.12443
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发表时间：
2016-12
影响因子：
2.9
通讯作者：
Takehiro Shinohara;T. Izawa;Akiko Mino-Oka;Hiroki Mori;Akihiko Iwasa;Toshihiro Inubushi;Yu Yamaguchi;Eiji Tanaka
中科院分区：
文献类型：
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作者：
Takehiro Shinohara;T. Izawa;Akiko Mino-Oka;Hiroki Mori;Akihiko Iwasa;Toshihiro Inubushi;Yu Yamaguchi;Eiji Tanaka
本研究旨在探讨透明质酸(HA)代谢与机械负荷所致的颞下颌关节骨关节炎(TMJ-OA)发生和发展的关系。将2月龄和6月龄C57BL/6N小鼠分为实验对照组和未治疗对照组(n=5/组)。将滑板固定在实验小鼠上颌骨门牙上10天，使TMJ的髁突软骨超载。实验组在苏木精-伊红、藏红-O-坚固绿染色切片上可见明显的软骨降解。研究还表明，在对照组和实验组中，老年小鼠的软骨降解程度都更大。机械超负荷后，HABP阳性细胞数随年龄增加而减少。HA表达的降低与机械超负荷所致软骨退化的进程有关。对各组透明质酸合成和降解相关基因的表达进行绝对定量。在幼龄小鼠中，实验组HA合成酶(HAS)2和3的mRNA表达水平低于对照组。相比之下，在老年小鼠中，实验组HA降解基因Hyal2和KIAA1199的mRNA表达水平高于对照组。因此，机械过载分别对老年和年轻小鼠的HA降解和HA合成的平衡产生不同的影响。综上所述，机械超负荷影响了HA的代谢，可能启动或放大了髁突软骨的退化。
This study aimed to examine hyaluronan (HA) metabolism in relation to the onset and progression of temporomandibular joint osteoarthritis (TMJ-OA) induced by mechanical overloading. Two-month-old and 6-month-old C57BL/6N mice were divided into experimental and untreated control groups (n = 5/group). A sliding plate was attached to the maxillary incisors of the experimental mice for 10 days to overload the condylar cartilage in TMJ. In experimental group, profound cartilage degradation was detected in haematoxylin-eosin, Safranin-O-Fast Green-stained sections. It was also shown that the cartilage degradation was greater in older mice in both the control and the experimental groups. The number of HABP-positive cells was decreased by mechanical overloading and with age. The reduction of HA expression was correlated with the progression of cartilage degradation induced by mechanical overloading. The absolute quantification of the mRNA expression related to HA synthesis and HA degradation was also performed in each group. The mRNA expression levels of HA synthase (HAS) 2 and 3 were lower in the experimental group compared with the control group in the younger mice. In contrast, the mRNA expression levels of the HA degradation gene, HYAL2 and KIAA1199, were higher in the experimental group compared with the control group in the older mice. Thus, mechanical overload differently affected the balance of HA degradation and HA synthesis in the older and younger mice, respectively. In conclusion, mechanical overloading affects HA metabolism and it might initiate or amplify the condylar cartilage degradation.