On the Ionization and Ion Transmission Efficiencies of Different ESI-MS Interfaces
On the Ionization and Ion Transmission Efficiencies of Different ESI-MS Interfaces
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DOI：
10.1007/s13361-014-0998-5
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发表时间：
2015-01-01
影响因子：
3.2
通讯作者：
Tang, Keqi
中科院分区：
文献类型：
--
作者：
Cox, Jonathan T.;Marginean, Ioan;Tang, Keqi
电喷雾电离质谱 (ESI-MS) 可实现的灵敏度很大程度上取决于 ESI 源的电离效率和通过 ESI-MS 接口的离子传输效率。这些性能特征很难跨多个平台进行评估和比较，因为很难将电流测量值与到达质谱仪检测器的实际分析物离子相关联。我们提出了一种有效的方法来评估 ESI-MS 接口的整体离子利用效率，方法是测量通过接口传输的总气相离子电流，并将其与相应质谱中测量的观察到的离子丰度相关联。利用这种方法，我们系统地研究了不同 ESI-MS 接口配置的离子传输和电离效率，包括单发射器/单入口毛细管、单发射器/多入口毛细管，以及分别具有单发射器和发射器阵列的纳电喷雾 (SPIN) MS 接口的负压电离。我们的实验结果表明，SPIN-MS 接口配置的总体离子利用效率超过了基于入口毛细管的 ESI-MS 接口配置。
The achievable sensitivity of electrospray ionization mass spectrometry (ESI-MS) is largely determined by the ionization efficiency in the ESI source and ion transmission efficiency through the ESI-MS interface. These performance characteristics are difficult to evaluate and compare across multiple platforms as it is difficult to correlate electrical current measurements to actual analyte ions reaching the detector of a mass spectrometer. We present an effective method to evaluate the overall ion utilization efficiency of an ESI-MS interface by measuring the total gas-phase ion current transmitted through the interface and correlating it to the observed ion abundance measured in the corresponding mass spectrum. Using this method, we systematically studied the ion transmission and ionization efficiencies of different ESI-MS interface configurations, including a single emitter/single inlet capillary, single emitter/multi-inlet capillary, and a subambient pressure ionization with nanoelectrospray (SPIN) MS interface with a single emitter and an emitter array, respectively. Our experimental results indicate that the overall ion utilization efficiency of SPIN-MS interface configurations exceeds that of the inlet capillary-based ESI-MS interface configurations.