The act of voluntary wheel running reverses dietary hyperphagia and increases leptin signaling in ventral tegmental area of aged obese rats.
The act of voluntary wheel running reverses dietary hyperphagia and increases leptin signaling in ventral tegmental area of aged obese rats.
复制标题
自愿跑轮的行为可以逆转老年肥胖大鼠的饮食过量并增加腹侧被盖区的瘦素信号传导。
DOI：
10.1159/000321343
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发表时间：
2011
期刊：
影响因子：
3.5
通讯作者：
Scarpace,PhilipJ
中科院分区：
文献类型：
--
作者：
Shapiro,Alexandra;Cheng,Kit-Yan;Gao,Yongxin;Seo,Dong-Oh;Anton,Steve;Carter,ChristyS;Zhang,Yi;Tumer,Nihal;Scarpace,PhilipJ
为了验证运动增加中枢瘦素信号传导，从而减少老年肥胖模型中的饮食体重增加的假设，我们评估了23个月大的F344×BN大鼠自愿轮跑（WR）的影响，喂食60%高脂（HF）饮食3个月。在HF饮食2个月后，一半的大鼠被提供2周的跑步轮，而另一半保持久坐不动。在移除轮子后，评估身体表现，4周后，在急性WR后评估下丘脑和VTA中的瘦素信号。HF饮食的引入导致长时间的摄食过多（普通饮食为63.9 ± 7.8 kcal/天，高脂肪饮食为88.1 ± 8.2 kcal/天（当食物摄入稳定时），p < 0.001）。每天少至9次（范围至135次）的轮旋转将HF食物的热量消耗（46.8 ± 11.2千卡/天）显著降低至低于普通饮食的水平（63.9 ± 7.8千卡/天，p < 0.001）。在2周的WR后，体重显著降低（与跑步前体重相比为7.9 ± 2.1%，p < 0.001），并且身体表现（从斜面跌落的潜伏期）显著改善（p = 0.04）。WR显著增加了腹侧被盖区（VTA）的基础（p = 0.04）和瘦素刺激（p = 0.001）STAT 3磷酸化，但在下丘脑中没有。因此，在老年饮食性肥胖大鼠中，自愿WR的行为而非程度在逆转HF消耗、降低体重和改善身体表现方面非常有效。它似乎引发了一种反应，取代了高可口食物的奖励，这可能是由腹侧被盖区瘦素信号传导增加介导的。
To test the hypothesis that exercise increases central leptin signaling, and thus reduces dietary weight gain in an aged obese model, we assessed the effects of voluntary wheel running (WR) in 23-month-old F344×BN rats fed a 60% high-fat (HF) diet for 3 months. After 2 months on the HF diet, half of the rats were provided access to running wheels for 2 weeks while the other half remained sedentary. Following the removal of the wheels, physical performance was evaluated, and 4 weeks later leptin signaling was assessed in hypothalamus and VTA after an acute bout of WR. Introduction of a HF diet led to prolonged hyperphagia (63.9 ± 7.8 kcal/day on chow diet vs. 88.1 ± 8.2 kcal/day on high-fat diet (when food intake stabilized), p < 0.001). As little as 9 (ranging to 135) wheel revolutions per day significantly reduced caloric consumption of HF food (46.8 ± 11.2 kcal/day) to a level below that on chow diet (63.9 ± 7.8 kcal/day, p < 0.001). After 2 weeks of WR, body weight was significantly reduced (7.9 ± 2.1% compared with prerunning weight, p < 0.001), and physical performance (latency to fall from an incline plane) was significantly improved (p = 0.04). WR significantly increased both basal (p = 0.04) and leptin-stimulated (p = 0.001) STAT3 phosphorylation in the ventral tegmental area (VTA), but not in the hypothalamus. Thus, in aged dietary obese rats, the act but not the extent of voluntary WR is highly effective in reversing HF consumption, decreasing body weight, and improving physical performance. It appears to trigger a response that substitutes for the reward of highly palatable food that may be mediated by increased leptin signaling in the VTA.