Some Phenolic Compounds Increase the Nitric Oxide Level in Endothelial Cells in Vitro
Some Phenolic Compounds Increase the Nitric Oxide Level in Endothelial Cells in Vitro
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DOI：
10.1021/jf901381x
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发表时间：
2009-09-09
影响因子：
6.1
通讯作者：
Hollman, Peter C. H.
中科院分区：
文献类型：
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作者：
Appeldoorn, Maaike M.;Venema, Dini P.;Hollman, Peter C. H.
巧克力和葡萄酒的血管舒张特性可能与酚类化合物的存在有关。所涉及的潜在机制之一是刺激内皮一氧化氮（NO）产生，因为NO是血管舒张的主要调节剂。本研究的目的是开发一种在体外测定使用杂交人内皮细胞系EA. hy 926快速筛选酚类化合物的NO刺激潜力。该测定进行了优化，并选择33酚，即，原花青素，单体黄烷-3-醇，黄酮醇，黄酮，黄烷酮，查耳酮，芪，和酚酸，测试他们的能力，以提高内皮NO水平。白藜芦醇，一种众所周知的NO水平的增强剂，被包括作为阳性对照。在所测试的33种酚类化合物中，只有白藜芦醇（NO水平增加285%）、槲皮素（增加110%）、表儿茶素没食子酸酯（ECg）（增加85%）和表没食子儿茶素没食子酸酯（EGCg）（增加60%）具有显著性（P
The vasorelaxing properties of chocolate and wine might relate to the presence of phenolic compounds. One of the potential mechanisms involved is stimulation of endothelial nitric oxide (NO) production, as NO is a major regulator of vasodilatation. This study aimed to develop an in vitro assay using the hybrid human endothelial cell line EA.hy926 to rapidly screen phenolic compounds for their NO-stimulating potential. The assay was optimized, and a selection of 33 phenolics, namely, procyanidins, monomeric flavan-3-ols, flavonols, a flavone, a flavanone, a chalcone, a stilbene, and phenolic acids, was tested for their ability to enhance endothelial NO level. Resveratrol, a well-known enhancer of NO level, was included as a positive control. Of the 33 phenolics tested, only resveratrol (285% increase in NO level), quercetin (110% increase), epicatechingallate (ECg) (85% increase), and epigallocatechingallate (EGCg) (60% increase) were significant (P