GINGER RHIZOME: A NEW SOURCE OF PROTEOLYTIC ENZYME
GINGER RHIZOME: A NEW SOURCE OF PROTEOLYTIC ENZYME
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生姜根茎：蛋白水解酶的新来源
DOI：
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
C. Allen
中科院分区：
文献类型：
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作者：
E. H. Thompson;I. D. Wolf;C. Allen
以牛血清白蛋白(BSA)、胶原和肌动球蛋白为底物，研究了生姜的蛋白水解性。通过对生姜根茎丙酮粉的缓冲提取和丙酮沉淀，制备了一种半纯化的粉末状酶制剂。以3%牛血清白蛋白为底物，在pH为4.5~6.0的范围内有较高的蛋白水解率，最适pH为5.0。BSA蛋白水解酶的最适反应温度为60℃，反应时间为10分钟，酶的快速变性发生在70℃，10%的NaC l使胶原蛋白和BSA蛋白水解率分别降低20%和50%。在提取和反应过程中，二硫苏糖醇对生姜蛋白水解酶有保护作用，说明活性部位有−-SH基团参与。对可溶性多肽、氨基酸或末端氨基酸的分析表明，胶原蛋白的蛋白水解率是肌动球蛋白的数倍。生姜蛋白水解酶对这两种肌肉蛋白质组分进行联合蛋白分解，可显著增加肉质的嫩度。根据传统的命名法，生姜是生姜或生姜中这种蛋白质分解活性成分的专有名称，通常被称为生姜。对于肉类应用，与木瓜酶和无花果酶相比，姜黄素的一个可能的优势是，与肌动球蛋白相比，胶原蛋白的蛋白分解程度更高。与菠萝酶的报道值相比，生姜具有更高的最适活性温度，这在某些应用中是可取的。
The proteolytic activity of ginger rhizome was studied with bovine serum albumin (BSA), collagen and actomyosin as substrates. A semipurified, powdered enzyme preparation was prepared by buffer extraction of an acetone powder of ginger rhizome and subsequent acetone precipitation of the proteolytic principle from the buffer extract. With 3% BSA as substrate, a relatively high proteolytic activity occurred over a pH range of 4.5–6.0, with an optimum pH of 5.0. The optimum temperature for proteolysis of BSA was 60°C during a 10 min reaction time, with rapid denaturation of the enzyme occurring at 70° C. NaCl in cone up to 10% produced about a 20 and 50% reduction in proteolysis of collagen and BSA, respectively. The ginger protease was protected by dithiothreitol during extraction and reaction, indicating the involvement of −SH groups at the active site. The analyses of soluble peptide amino acids or terminal amino acids suggest that the proteolysis of collagen is many fold greater than that of actomyosin. The combined proteolysis of these two muscle protein fractions by the ginger protease resulted in significantly more tender meat. According to conventional nomenclature, “Zingibain” is the proper name for this proteolytically active principle in Zingiber officinale roscoe or ginger rhizome which is commonly referred to as gingerroot. For meat applications, a possible advantage of zingibain over papain and ficin is the greater proteolysis of collagen in comparison to actomyosin. When compared to reported values for bromelain, zingibain has a higher optimum activity temperature, which is desirable in some applications