Sonication is superior to scraping for retrieval of bacteria in biofilm on titanium and steel surfaces in vitro.
Sonication is superior to scraping for retrieval of bacteria in biofilm on titanium and steel surfaces in vitro.
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DOI：
10.3109/17453670902947457
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发表时间：
2009-04
影响因子：
3.7
通讯作者：
Bergh K
中科院分区：
文献类型：
--
作者：
Bjerkan G;Witsø E;Bergh K
背景和目的 低毒力植入物感染的特征是植入物的细菌定殖以及随后的生物膜形成。在这些情况下，软组织活检通常证明培养阴性。因此，致病粘附细菌的分离对于正确的微生物学诊断至关重要。使用体外模型，我们比较了从金属表面采集生物膜的 4 种方法。方法 将钛和钢圆盘在金黄色葡萄球菌、表皮葡萄球菌、粪肠球菌和痤疮丙酸杆菌存在的 Mueller Hinton 肉汤中培养。通过反复冲洗圆盘去除未粘附的细菌。对每个圆盘的 10 个平行样进行 4 种细菌回收方法中的一种：(A) 对圆盘进行超声处理，(B) 使用手术刀片刮擦圆盘，然后将刀片划到琼脂板上，(C) 刮擦圆盘，然后对手术刀片进行涡流混合，以及 (D) 刮擦圆盘，然后对手术刀片进行超声处理。对每种取样方法进行定量细菌培养。结果 除了钢上的表皮葡萄球菌外，超声处理可以有效且可靠地去除生物膜细菌。所采用的刮擦方法没有分离嵌入生物膜中的细菌。解释 刮擦金属表面并不是体外生物膜细菌取样的适当方法。
Background and purpose Low-virulence implant infections are characterized by bacterial colonization of the implant with subsequent biofilm formation. In these cases, soft tissue biopsies often prove to be culture negative. Consequently, detachment of the causative adherent bacteria is crucial for correct microbiological diagnosis. Using an in vitro model, we compared 4 methods of biofilm sampling from metal surfaces. Methods Discs of titanium and steel were incubated in the presence of Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, and Propionibacterium acnes in Mueller Hinton broth. Non-adherent bacteria were removed by repeated rinsing of the discs. 10 parallels of each disc were subjected to 1 of 4 methods for bacterial recovery: (A) sonication of the discs, (B) scraping of the discs using surgical blades followed by streaking of the blades onto agar plates, (C) scraping of the discs followed by vortex mixing of the surgical blades, and (D) scraping of the discs followed by sonication of the surgical blades. Quantitative bacterial cultures were performed for each sampling method. Results With the exception of S. epidermidis on steel, sonication efficiently and reliably dislodged biofilm bacteria. The scraping methods employed did not detach bacteria embedded in biofilm. Interpretation Scraping of metal surfaces is not an adequate method for sampling of biofilm bacteria in vitro.