Abnormal erythroid differentiation in neonatal bcl-6-deficient mice
Abnormal erythroid differentiation in neonatal bcl-6-deficient mice
复制标题
DOI：
10.1016/j.exphem.2004.10.001
复制
发表时间：
2005-01-01
影响因子：
2.6
通讯作者：
Tokuhisa, T
中科院分区：
文献类型：
--
作者：
Asari, S;Sakamoto, A;Tokuhisa, T
客观的。 bcl-6原癌基因在多种组织中普遍表达。由于我们发现新生bcl-6缺陷(bcl-6(-/-))小鼠的脾脏中TER119(+)细胞数量少于对照(bcl-6(+/+))同窝小鼠，因此我们研究了bcl-6在红系细胞分化中的功能。 材料和方法。使用抗 TER119 和抗 CD71 mAb 将最终红细胞生成中的成红细胞分为四个亚群。使用流式细胞仪检测新生 bcl-6(+/+) 和 bcl-6(-/-) 小鼠脾脏中这四个亚群的细胞数量和性质。结果。通过逆转录聚合酶链反应，在形态学上与嗜碱性成红细胞相当的 TER119(high) CD71(high) 亚群中检测到 bcl-6 mRNA 表达。 TER119（低）CD71（高）和TER119（高）CD71（高）亚群中的细胞处于细胞周期中的比例很高。新生bcl-6(-/-)小鼠脾脏TER119(high)CD71(high)亚群细胞数和外周血网织红细胞百分比均显着低于新生bcl-6(+/+)小鼠。而bcl-6(-/-)小鼠TER119(high)CD71(high)亚群的凋亡细胞百分比和处于细胞周期的细胞百分比与bcl-6(+/+)小鼠相似。结论。在新生小鼠脾脏中的 TER119（高）CD71（高）成红细胞亚群中检测到的 bcl-6 可能需要将成红细胞保留在细胞增殖阶段。 (C) 2005 年国际实验血液学学会。由爱思唯尔公司出版
Objective. The bcl-6 proto-oncogene is ubiquitously expressed in various tissues. Since we found out the smaller number of TER119(+) cells in the spleen of neonatal bcl-6-deficient (bcl-6(-/-)) mice compared with that of control (bcl-6(+/+)) littermates, we studied functions of bcl-6 in differentiation of erythroid lineage cells.Materials and Methods. Erythroblasts in the definitive erythropoiesis were separated into four subsets using anti-TER119 and anti-CD71 mAbs. The cell number and property of these four subsets in spleens of neonatal bcl-6(+/+) and bcl-6(-/-) mice were examined using a flow cytometry.Results. bcl-6 mRNA expression was detected in the TER119(high) CD71(high) subset, which is morphologically equivalent to basophilic erythroblasts, by reverse-transcribed polymerase chain reaction. High percentages of cells in the TER119(low)CD71(high) and TER119(high)CD71(high) subsets were in the cell cycle. The cell number of the TER119(high)CD71(high) subset in the spleen and the percentage of reticulocytes in the peripheral blood of neonatal bcl-6(-/-) mice were significantly lower than those of neonatal bcl-6(+/+) mice. However, the percentage of apoptotic cells and that of cells in the cell cycle in the TER119(high)CD71(high) subset of bcl-6(-/-) mice were similar to those of bcl-6(+/+) mice.Conclusion. bcl-6 detected in the TER119(high)CD71(high) subset of erythroblasts in the spleen of neonatal mice may be required to retain the erythroblasts in the cell proliferation stage. (C) 2005 International Society for Experimental Hematology. Published by Elsevier Inc.