Genetic variation and aiffinity of Cordyceps sinensis in Qinghai Province based on analysis of morphologic characters and inter-simple sequence repeat markers
Genetic variation and aiffinity of Cordyceps sinensis in Qinghai Province based on analysis of morphologic characters and inter-simple sequence repeat markers
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发表时间：
2005
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通讯作者：
Cheng-Yan Zhou
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作者：
Cheng-Yan Zhou
目的对青海省中国不同产地冬虫夏草进行鉴定，估计其遗传变异、亲缘关系和区域分布。方法对青海省11个县市的中华绒毛虫标本进行形态特征和ISSR标记分析。结果中华绒毛虫在形态特征和ISSR多态性上均存在明显的遗传变异。形态分析数据表明，这些中华绒毛虫标本大致可分为三类，分别分布在青海省的青海湖、中东和青海省南部地区。然而，仅凭形态特征对特定样品进行鉴定是困难和含糊的。根据ISSR标记分析，青海省中华绒毛虫可分为两大类群。其中一个样本来自环青海湖地区，另一个来自其他两个地区，其中一个样本可进一步划分为三个亚组，分别与青海省南部、中部和东部地区的不同生境有很好的相关性。结论青海省华支睾吸虫的遗传变异和亲缘关系因其生活环境而广泛分布。ISSR分析是鉴定中华绒毛虫样品和确定其遗传亲缘关系的有效工具，特别是结合形态特征分析。
Objective To identify Cordyceps sinensis from different habitats in Qinghai Province of China and estimate their genetic variation, affinity, and regional distribution. Methods Morphological characters and inter-simple sequence repeat (ISSR) markers were analyzed among the C. sinensis samples from 11 Counties in Qinghai Province. Results Significant genetic variations were detected in both morphological characters and ISSR polymorphisms among these C. sinensis samples. Data from morphological analysis showed that these C. sinensis samples could be approximately divided into three groups, which distributed around the Qinghai Lake, middle east, and south areas in Qinghai Province, respectively. However it was arduous and ambiguous to identify the specific samples only by morphological characters. Based on the analysis of ISSR markers, C. sinensis of Qinghai Province could be classified into two major groups. One included the samples from the areas around the Qinghai Lake and another from the other two areas, one of which could be further divided into three subgroups, well correlated with the different habitats of south, center, and east areas in Qinghai Province, respectively. Conclusion C. sinensis with genetic variation and affinity in Qinghai Province distributes widely according to their living environment. ISSR analysis is an effective tool in identifying C. sinensis samples and determining their genetic affinity particularly when combined with the analysis of morphological characters.