Direct and sex-specific stimulation by sex steroids of creatine kinase activity and DNA synthesis in rat bone.
Direct and sex-specific stimulation by sex steroids of creatine kinase activity and DNA synthesis in rat bone.
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性类固醇对大鼠骨骼肌酸激酶活性和 DNA 合成的直接和性别特异性刺激。
DOI：
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发表时间：
1989
影响因子：
11.1
通讯作者：
A. Kaye
中科院分区：
文献类型：
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作者：
D. Somjen;Y. Weisman;A. Harell;E. Berger;A. Kaye
17 β-雌二醇（E2）在体外对骨和软骨细胞能量代谢的直接作用已得到证实。E2和睾酮对断乳大鼠骨干骨的刺激具有性别特异性。E2（30 nM）在24小时内导致ROS 17/2.8大鼠成骨肉瘤细胞、MC 3 T3-E1小鼠颅骨衍生细胞和大鼠胎儿颅骨细胞中肌酸激酶（CK; ATP：肌酸N-磷酸转移酶，EC2.7.3.2）比活性增加70-200%，并导致大鼠骺软骨细胞中肌酸激酶比活性增加40%。E2对CK活性的刺激具有剂量和时间依赖性：在ROS 17/2.8细胞中，在3 nM E2时发现CK活性显著增加，在E2给药后1小时发现CK活性增加超过100%。在雌性20日龄Wistar大鼠中，E2（每只大鼠5微克）在腹腔注射后1小时内使骨干骨中CK活性增加82%，24小时后最大增加200%;弱雌激素激动剂17 α-雌二醇、睾酮和孕酮均未显示出这种作用。相反，在雄性大鼠骨干骨，睾酮或双氢睾酮增加CK活性后24小时约100%，而E2是无效的。在骺软骨，E2和睾酮增加CK活性。性激素对CK活性的刺激被[3 H]胸苷掺入DNA的显著增加所抵消。因此，性腺类固醇的直接性别特异性作用可能有助于刺激骨生长和维持平衡的骨转换。
A direct in vitro effect of 17 beta-estradiol (E2) was demonstrated on bone and cartilage cell energy metabolism. Sex-specific stimulation by E2 and testosterone was shown in diaphyseal bone of weanling rats. E2 (30 nM) caused, within 24 hr, a 70-200% increase in creatine kinase (CK; ATP:creatine N-phosphotransferase, EC 2.7.3.2) specific activity in ROS 17/2.8 rat osteogenic sarcoma cells, MC3T3-E1 mouse calvaria-derived cells, and rat fetal calvaria cells, and a 40% increase in rat epiphyseal cartilage cells. Stimulation of CK activity by E2 was dose and time dependent: in ROS 17/2.8 cells, a highly significant increase was found at 3 nM E2 and a greater than 100% increase in CK activity was found 1 hr after E2 administration. In female 20-day-old Wistar-derived rats, E2 (5 micrograms per rat) increased CK activity in diaphyseal bone by 82% within 1 hr of i.p. injection, with a maximal increase of 200% after 24 hr; neither the weakly estrogenic agonist 17 alpha-estradiol, testosterone, nor progesterone showed this effect. Conversely, in male rat diaphyseal bone, testosterone or dihydrotestosterone increased CK activity after 24 hr by approximately 100%, while E2 was ineffective. In epiphyseal cartilage, both E2 and testosterone increased CK activity. Stimulation of CK activity by sex hormones was paralleled by significant increases in [3H]thymidine incorporation into DNA. Therefore, it is possible that direct sex-specific actions of gonadal steroids may contribute to stimulating bone growth and maintaining balanced bone turnover.