Overexpression of AtALMT1 in the Arabidopsis thaliana ecotype Columbia results in enhanced Al-activated malate excretion and beneficial bacterium recruitment.
Overexpression of AtALMT1 in the Arabidopsis thaliana ecotype Columbia results in enhanced Al-activated malate excretion and beneficial bacterium recruitment.
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DOI：
10.4161/psb.25565
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发表时间：
2013-09
影响因子：
2.9
通讯作者：
Koyama H
中科院分区：
文献类型：
--
作者：
Kobayashi Y;Lakshmanan V;Kobayashi Y;Asai M;Iuchi S;Kobayashi M;Bais HP;Koyama H
AtALMT1（Arabidopsis thaliana ALuminum activated Malate Transporter 1）编码一种在拟南芥抗逆中具有多效性的拟南芥苹果酸转运蛋白。通过AtALMT1释放的苹果酸保护根尖免受Al根毒，并招募有益的根际细菌诱导植物免疫。为了检验AtALMT 1的过表达是否可以改善这些性状，将由花椰菜花叶病毒35 S启动子驱动的基因引入拟南芥生态型哥伦比亚。该基因的过表达增强铝激活的苹果酸排泄和有益细菌枯草芽孢杆菌菌株FB17的招聘。这些研究结果表明，AtALMT1的过表达可以被用来作为一种方法，以提高植物的能力，释放苹果酸到根际，这可以提高植物对一些环境胁迫因素的耐受性。
AtALMT1 (Arabidopsis thaliana ALuminum activated Malate Transporter 1) encodes an Arabidopsis thaliana malate transporter that has a pleiotropic role in Arabidopsis stress tolerance. Malate released through AtALMT1 protects the root tip from Al rhizotoxicity, and recruits beneficial rhizobacteria that induce plant immunity. To examine whether the overexpression of AtALMT1 can improve these traits, the gene, driven by the cauliflower mosaic virus 35S promoter, was introduced into the Arabidopsis ecotype Columbia. Overexpression of the gene enhanced both Al-activated malate excretion and the recruitment of beneficial bacteria Bacillus subtilis strain FB17. These findings suggest that overexpression of AtALMT1 can be used as an approach to enhance a plant's ability to release malate into the rhizosphere, which can enhance plant tolerance to some environmental stress factors.