Increased release of myeloperoxidase in vitro from neutrophils of patients with myeloperoxidase specific anti-neutrophil cytoplasmic antibody (MPO-ANCA) related glomerulonephritis
Increased release of myeloperoxidase in vitro from neutrophils of patients with myeloperoxidase specific anti-neutrophil cytoplasmic antibody (MPO-ANCA) related glomerulonephritis
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DOI：
10.1111/j.1440-1797.1997.tb00237.x
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发表时间：
1997-12-01
期刊：
影响因子：
2.5
通讯作者：
Suzuki, K
中科院分区：
文献类型：
--
作者：
Minoshima, S;Arimura, Y;Suzuki, K
为探讨中性粒细胞在MPO特异性抗中性粒细胞胞浆抗体(MPO ANCA)相关性肾小球肾炎(GN)发病机制中的作用，对MPO相关GN患者中性粒细胞MPO释放、β-葡萄糖醛酸酶(BGL)释放、超氧阴离子(O-2(-))产生进行了检测。同时研究了血浆对MPO-ANCA相关性肾炎患者中性粒细胞释放MPO的影响。采集MPO ANCA相关性GN、与MPO-ANCA无关的GN患者和健康对照组的中性粒细胞和血浆。MPO-ANCA相关性肾炎患者中性粒细胞释放MPO明显高于对照组。这也高于与MPO-ANCA无关的肾炎患者，但这在统计学上没有统计学意义。MPO-ANCA相关性肾炎患者中性粒细胞产生超氧阴离子明显高于非MPO-ANCA相关性肾炎患者。然而，BGL的释放在三组之间没有显著差异。此外，MPO释放和O-2(-)产生与MPO-ANCA相关的GN的临床活动平行增加。MPO-ANCA相关性肾炎患者中性粒细胞对FMLP释放MPO的敏感性明显高于其他两组。与MPO-ANCA相关的肾小球肾炎患者的血浆可增加中性粒细胞对FMLP释放MPO的敏感性，但对BGL的释放无明显影响，而对MPO-ANCA相关肾炎患者的中性粒细胞释放MPO有抑制作用。提示：在MPO-ANCA患者中，中性粒细胞可高度释放MPO，且MPO-ANCA患者血浆中含有调节中性粒细胞活性的S因子。
In order to elucidate the role of neutrophils in the pathogenesis of MPO-specific anti-neutrophil cytoplasmic antibody (MPO-ANCA) related glomerulonephritis (GN), MPO release, beta-glucuronidase (BGL) release, superoxide anion (O-2(-)) production from the neutrophils of patients with MPO-ANCA related GN were measured. The effect of plasma on MPO release from neutrophils was also studied in patients with MPO-ANCA related GN. Neutrophils and plasma were obtained from patients with MPO ANCA related GN, GN unrelated to MPO-ANCA and healthy controls. MPO release from the neutrophils of patients with MPO-ANCA related GN was higher than that of controls significantly. This was also higher than that in patients with GN unrelated to MPO-ANCA, but this was not statistically significant. Superoxide anion production from neutrophils of patients with MPO-ANCA related GN was significantly higher than that in patients with GN unrelated MPO-ANCA. However, BGL release was not significantly different among three groups. Furthermore, MPO release and O-2(-) production increased in parallel with clinical activity of MPO-ANCA related GN. Neutrophils of patients with MPO-ANCA related GN showed to be significantly more sensitive to FMLP on MPO release than those in the other two groups. However, plasma from MPO-ANCA related GN increased the sensitivity to FMLP on MPO release, but not BGL release, in neutrophils obtained from healthy controls, whereas it suppressed MPO release from neutrophils with MPO-ANCA related GN. This suggests;that in patients with MPO-ANCA related GN MPO can be highly released from activated neutrophils and that the plasma of patients with MPO-ANCA contains factor(s) which modulate reactivity of neutrophils.