Degradation of single- and double-stranded RNA by frog virus 3.
Degradation of single- and double-stranded RNA by frog virus 3.
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青蛙病毒3对单链和双链RNA的降解。
DOI：
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发表时间：
1972
影响因子：
11.1
通讯作者：
G. Koch
中科院分区：
文献类型：
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作者：
P. Palese;G. Koch
青蛙病毒 3 的纯化制剂具有针对单链和双链 RNA 的核糖核酸酶活性。从纯化的 3 型呼肠孤病毒和感染脊髓灰质炎病毒的 HeLa 细胞中分离出的双链 RNA，以及从纯化病毒中分离出的单链脊髓灰质炎病毒 RNA，通过与青蛙病毒 3 一起孵育，很容易降解。核酸酶的作用方式是核酸内切。在用于病毒酶的测定条件下，未感染的 HeLa、L 和幼仓鼠肾细胞的粗提物没有表现出针对双链 RNA 的酶活性，但表现出针对单链 RNA 的活性。病毒核酸酶对二价阳离子的最佳活性依赖以及 0.2 M NaCl 对单链 RNA 裂解的抑制表明这些酶要么是病毒编码的，要么是病毒诱导的。
Purified preparations of frog virus 3 possess ribonuclease activities directed against single-and double-stranded RNA. Double-stranded RNAs isolated from purified reovirus type 3 and from HeLa cells infected with poliovirus and single-stranded poliovirus RNA from purified virus are readily degraded by incubation with frog virus 3. The mode of action of the nucleases is endonucleolytic. Under the assay conditions used for the viral enzyme, crude extracts of uninfected HeLa, L, and baby hamster kidney cells did not show enzyme activity against double-stranded RNA but exhibited activity against single-stranded RNA. The dependence of the viral nucleases on divalent cations for optimal activity and the inhibition of the cleavage of single-stranded RNA by 0.2 M NaCl suggests that the enzymes are either virus-coded or virus-induced.