An efficient DDAB-mediated transfection of Drosophila S2 cells

An efficient DDAB-mediated transfection of Drosophila S2 cells
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DOI:
10.1093/nar/24.21.4362
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发表时间:
1996-11-01
影响因子:
14.9
通讯作者:
Han, KH
Han, KH
中科院分区:
生物学2区
文献类型:
--
作者:
Han, KH

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我开发了一种使用阳离子脂质体试剂 DDAB 转染果蝇 S2 细胞的有效方法。与传统的磷酸钙方法相比,优化的DDAB方法使转染效率提高了10倍或更多,而传统的磷酸钙方法基本上是转染S2细胞的唯一方法。
I have developed an efficient method for transfecting Drosophila S2 cells using DDAB, a cationic liposome reagent. The optimized DDAB method resulted in a 10 times or greater increase in transfection efficiency compared with the conventional calcium phosphate method which has been essentially the only way for transfecting S2 cells.