PULSED-FIELD GEL-ELECTROPHORESIS AS A REPLACEMENT FOR BACTERIOPHAGE-TYPING OF STAPHYLOCOCCUS-AUREUS
PULSED-FIELD GEL-ELECTROPHORESIS AS A REPLACEMENT FOR BACTERIOPHAGE-TYPING OF STAPHYLOCOCCUS-AUREUS
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DOI：
10.1128/jcm.33.3.551-555.1995
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发表时间：
1995-03-01
影响因子：
9.4
通讯作者：
MILLER, JM
中科院分区：
文献类型：
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作者：
BANNERMAN, TL;HANCOCK, GA;MILLER, JM
噬菌体分型（BT）（世界卫生组织方法）已在疾病控制和预防中心使用了30多年，用于对金黄色葡萄球菌分离株进行分型。由于研究表明BT模式的重现性较差，并且由于BT未能对高比例（15%至20%）的分离株进行分型，因此疾病控制和预防中心已从使用BT转换为使用脉冲场凝胶电泳（PFGE）进行菌株分型。对300株金黄色葡萄球菌进行了BT和PFGE分型。金黄色葡萄球菌，包括来自几个特征良好的爆发的菌株。96株为BT I组，19株为II组，82株为III组，7株为V组，96株为不可分型。PFGE鉴定了每个噬菌体组内的亚组，因此比BT更具鉴别力，BT未鉴定亚组。PFGE能够对所有分离株进行分型，并区分相关和不相关的S。金黄色。我们改良的标准化PFGE方法应能使分型实验室从琼脂培养基上的分离菌落开始，在3 ~ 4天内获得快速、可靠的结果。
Bacteriophage typing (BT) (World Health Organization method) has been used at the Centers far Disease Control and Prevention for over 30 years to type isolates of Staphylococcus aureus. Since studies have shown that BT patterns have poor reproducibility and because BT fails to type a high percentage (15 to 20%) of isolates, the Centers for Disease Control and Prevention has converted from using BT to using pulsed-field gel electrophoresis (PFGE) for strain typing S. aureus, We compared the results of BT with results of PFGE for typing 300 isolates of S. aureus, including strains from several well-characterized outbreaks. Ninety-six isolates were BT group I, 19 were group II, 82 were group III, 7 were group V, and 96 were nontypeable. PFGE identified subgroups within each phage group and thus was more discriminating than BT, which identified no subgroups. PFGE was able to type all isolates and distinguish related from unrelated strains of S. aureus. Our modified, standardized PFGE methodology should enable typing laboratories to obtain rapid, reliable results in 3 to 4 days when starting with an isolated colony on agar media.