A scanning electron microscopic study of liver microcirculation disarrangement in experimental rat cirrhosis
A scanning electron microscopic study of liver microcirculation disarrangement in experimental rat cirrhosis
复制标题
实验性肝硬化大鼠肝脏微循环紊乱的扫描电镜研究
作者：
E. Gaudio;L. Pannarale;P. Onori;O. Riggio
多项研究表明肝脏微循环与肝功能有关。这种关系的原理在于血液从肝腺泡的供血血管到中央静脉的顺序分布。本研究旨在通过腐蚀铸件的光学显微镜和扫描电子显微镜研究发展中的肝硬化中肝脏微血管床不同部位的进行性变化。用胃内四氯化碳诱导实验性肝硬化。逐渐观察到的最重要的血管变化是正弦曲线前和正弦曲线后血管之间距离的减小、新形成的绕过正弦曲线的分流血管的存在，以及最后由正弦曲线前后血管组成的结节周围血管丛的发育。新形成的血管穿过预先形成的组织隔膜生长。这些血管的改变使得任何区域梯度几乎不可能实现。灌注分区梯度的丧失可能会严重改变肝功能以及肝层的结构变化。肝细胞再生无法恢复原有的血管关系：这使得肝硬化肝脏的形态和功能解构不可逆转。 （肝病学 1993；17：477–485。）
Hepatic microcirculation has been related to liver function in several studies. The principle of this relationship lies in the sequential distribution of blood from the feeding vessels of the hepatic acinus to the central vein. This study was undertaken to investigate the progressive changes at different sites of the liver microvascular bed in the developing cirrhosis, both by light microscopy and scanning electron microscopy of corrosion casts. Experimental cirrhosis was induced with intragastric carbon tetrachloride. The most important vascular changes progressively observed are the reduction of the distance between the pre‐ and postsinusoidal vessels, the presence of newly formed shunting vessels bypassing the sinusoids and, finally, the development of a perinodular vascular plexus composed of pre‐ and postsinusoidal vessels. Newly formed vessels grow through preformed tissue septa. These vascular modifications make any zonal gradient hardly possible. The loss of the zonal gradient of perfusion could highly modify liver function, along with the structural changes of hepatic laminae. Hepatocyte regeneration cannot recover the original vascular relationships: this makes the morphological and functional destructuralization of cirrhotic liver irreversible. (HEPATOLOGY 1993;17:477–485.)