EFFICIENT NEUTRALIZATION OF PRIMARY ISOLATES OF HIV-1 BY A RECOMBINANT HUMAN MONOCLONAL-ANTIBODY
EFFICIENT NEUTRALIZATION OF PRIMARY ISOLATES OF HIV-1 BY A RECOMBINANT HUMAN MONOCLONAL-ANTIBODY
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DOI：
10.1126/science.7973652
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发表时间：
1994-11-11
期刊：
影响因子：
56.9
通讯作者：
BARBAS, CF
中科院分区：
文献类型：
--
作者：
BURTON, DR;PYATI, J;BARBAS, CF
抗体在体外中和1型人类免疫缺陷病毒多种原始分离株的能力受到了质疑，这对疫苗可能的效力有影响。一种针对包膜糖蛋白gp120的重组人抗体被制备出来，并用于证明原始分离株并非对抗体中和有抗性。该重组抗体在通过被动免疫（例如阻断病毒的母婴传播）可达到的浓度下，能中和超过75%的受试原始分离株。这种抗体广泛的特异性和效力意味着gp120上一种结构特征的保守性，这在疫苗设计中可能很重要。
The ability of antibodies to neutralize diverse primary isolates of human immunodeficiency virus-type 1 in vitro has been questioned, with implications for the likely efficacy of vaccines. A recombinant human antibody to envelope glycoprotein gp120 was generated and used to show that primary isolates are not refractory to antibody neutralization. The recombinant antibody neutralized more than 75 percent of the primary isolates tested at concentrations that could be achieved by passive immunization, for example, to interrupt maternal-fetal transmission of virus. The broad specificity and efficacy of the antibody implies the conservation of a structural feature on gp120, which could be important in vaccine design.