Effects of acupuncture at HT7 on glucose metabolism in a rat model of Alzheimer's disease: an 18F-FDG-PET study.
Effects of acupuncture at HT7 on glucose metabolism in a rat model of Alzheimer's disease: an 18F-FDG-PET study.
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HT7 针刺对阿尔茨海默病大鼠模型葡萄糖代谢的影响：一项 18F-FDG-PET 研究。
DOI：
10.1136/acupmed-2015-010865
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Nie B
中科院分区：
文献类型：
--
作者：
Lai X;Ren J;Lu Y;Cui S;Chen J;Huang Y;Tang C;Shan B;Nie B
应用18f -2-氟-脱氧-d -葡萄糖正电子发射断层扫描（FDG-PET）探讨针刺HT7对阿尔茨海默病（AD）大鼠模型大脑不同区域的影响。60只Wistar大鼠接受了y迷宫电敏感性测试。10只大鼠作为健康对照组。其余50只大鼠在右侧大细胞基底核立体定向注射伊博特酸，并腹腔注射d -半乳糖。36只大鼠成功造模AD，随机分为3组（每组n=12）： AD组不给予治疗；AD+HT7组，在1个月内接受HT7穴位20次针刺；AD+Sham组，在远处的非穴位接受针灸。采用Y-maze法测定总反应时间（TRT），并于第1天和第30天进行18F-FDG-PET扫描。PET图像采用Statistical Parametric Mapping 8.0进行处理。治疗前，所有AD组的TRT均高于对照组（平均±SD为24.10±2.48 vs 41.34±5.00 s）。治疗后，与AD+Sham和AD组相比，AD+HT7组TRT缩短（p<0.0001，双向方差分析）。与健康对照相比，AD大鼠海马、丘脑、下丘脑、额叶和颞叶的葡萄糖代谢活性降低，HT7针刺后相对升高。与假针相比，HT7针刺对脑糖代谢有更大的正向影响。针刺HT7可提高AD大鼠海马、丘脑、下丘脑和额颞叶的记忆能力和脑葡萄糖代谢活性。
To explore the effects of acupuncture at HT7 on different cerebral regions in a rat model of Alzheimer's disease (AD) with the application of 18F-2-fluoro-deoxy-D-glucose positron emission tomography (FDG-PET). Sixty Wistar rats were included after undergoing a Y-maze electric sensitivity test. Ten rats were used as a healthy control group. The remaining 50 rats were injected stereotaxically with ibotenic acid into the right nucleus basalis magnocellularis and injected intraperitoneally with D-galactose. AD was successfully modelled in 36 rats, which were randomly divided into three groups (n=12 each): the AD group, which remained untreated; the AD+HT7 group, which received 20 sessions of acupuncture at HT7 over 1 month; and the AD+Sham group, which received acupuncture at a distant non-acupuncture point. Total reaction time (TRT) was measured by Y-maze and 18F-FDG-PET scans were conducted on day 1 and 30. PET images were processed with Statistical Parametric Mapping 8.0. Pre-treatment, TRT was greater in all AD groups versus controls (mean±SD 24.10±2.48 vs 41.34±5.00 s). Post-treatment, TRT was shortened in AD+HT7 versus AD+Sham and AD groups (p<0.0001, two-way analysis of variance). Glucose metabolic activity in the hippocampus, thalamus, hypothalamus, frontal lobe, and temporal lobe was decreased in AD rats compared with healthy controls and relatively elevated after HT7 acupuncture. Compared with sham acupuncture, HT7 needling had a greater positive influence on brain glucose metabolism. Needling at HT7 can improve memory ability and cerebral glucose metabolic activity of the hippocampus, thalamus, hypothalamus, and frontal/temporal lobes in an AD rat model.