Drosophila mitotic domain boundaries as cell fate boundaries
Drosophila mitotic domain boundaries as cell fate boundaries
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DOI：
10.1126/science.277.5327.825
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发表时间：
1997-08-08
期刊：
影响因子：
56.9
通讯作者：
Minden, JS
中科院分区：
文献类型：
--
作者：
Cambridge, SB;Davis, RL;Minden, JS
果蝇胚胎中有丝分裂区域的出现证明了命运的决定。为了确定细胞的命运，在有丝分裂结构域2、8和15中的单个细胞在发育过程中被标记和监测。不同命运的比较表明，区域边界是细胞命运边界。细胞被GAL4VP16类似物光激活后，GAL4VP16类似物的DNA结合活性被光致性阻断剂抑制，细胞被标记为gal4依赖性转基因的表达。笼化的GAL4VP16也被用来诱导爪蟾胚胎的基因表达。因此，光激活基因表达是一种多功能的基因表达时空控制工具。
Fate determination in Drosophila embryos is evidenced by the appearance of mitotic domains. To identify fate or fates of cells, individual cells in mitotic domains 2, 8, and 15 were marked and monitored through development. Comparison of the different fates indicated that domain boundaries are cell fate boundaries. Cells were marked by expression of GAL4-dependent transgenes after photoactivation of a caged GAL4VP16 analog that had its DNA binding activity inhibited with a photolabile blocking reagent. Caged GAL4VP16 was also used to induce gene expression in Xenopus embryos. Thus, photoactivated gene expression is a versatile tool for spatiotemporal control of gene expression.