Ceratocystis species infecting stem wounds on Eucalyptus grandis in South Africa
Ceratocystis species infecting stem wounds on Eucalyptus grandis in South Africa
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DOI：
10.1111/j.0032-0862.2004.01014.x
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发表时间：
2004-08-01
期刊：
影响因子：
2.7
通讯作者：
Wingfield, MJ
中科院分区：
文献类型：
--
作者：
Roux, J;van Wyk, M;Wingfield, MJ
长喙壳属的报道引起外来种植园阔叶树种的病害近年来有所增加。流苏长喙壳菌是桉树的主要病害之一。在非洲和南美洲，C.在非洲黑荆树（Acacia mearnsii）中，albofundus可导致黑荆树的枯萎和死亡。长喙壳属通常感染树木的伤口，因此人工茎伤口可以用来确定这些真菌的存在。本研究的目的是鉴定Ceratocystis spp.感染南非巨桉的伤口。分离的长喙壳属的形态特征和比较的DNA序列数据的ITS和5.8S区域的rRNA操纵子。在温室中进行致病性试验，以确定这些长喙壳属的可能作用。对疾病发展的影响这些试验也在田间条件下进行。三种长喙壳属收集到：C. fimbriata、毛唇隐翅虫C. moniliformis和C.梨形。这是C. fimbriata和C.梨形（pirilliformis）来自桉树属（Eucalyptus spp.）在南非，后者真菌在澳大利亚以外的第一次报告。两个C. fimbriata和C.梨形线虫对接种的E.巨树这是C.梨形菌是桉树的一种病原菌。从温室和田间试验的结果来看，它有可能在桉树人工林中引起严重的疾病问题。
Reports of Ceratocystis spp. causing disease of exotic plantation hardwood species have increased in recent years. Ceratocystis fimbriata causes wilt and canker on Eucalyptus spp. in Africa and South America, and C. albofundus results in wilt and death of Acacia mearnsii in Africa. Ceratocystis spp. generally infect wounds on trees, and artificial stem wounding can thus be used to determine the presence of these fungi. The aim of this study was to identify Ceratocystis spp. infecting wounds on Eucalyptus grandis in South Africa. Isolated Ceratocystis spp. were identified using morphological characteristics and comparisons of DNA sequence data for the ITS and 5.8S regions of the rRNA operon. Pathogenicity trials were conducted in the greenhouse to determine the possible role that these Ceratocystis spp. could have in disease development. These trials were also conducted under field conditions. Three Ceratocystis spp. were collected: C. fimbriata, C. moniliformis and C. pirilliformis. This is the first report of C. fimbriata and C. pirilliformis from Eucalyptus spp. in South Africa, and the first report of the latter fungus outside Australia. Both C. fimbriata and C. pirilliformis caused significant lesions on inoculated E. grandis trees. This is the first evidence that C. pirilliformis is a pathogen of Eucalyptus spp. From the results of both greenhouse and field trials, it has the potential to cause serious disease problems in Eucalyptus plantations.