Microscale Thermophoresis and additional effects measured in NanoTemper Monolith instruments
Microscale Thermophoresis and additional effects measured in NanoTemper Monolith instruments
复制标题
DOI：
10.1007/s00249-021-01529-1
复制
发表时间：
2021-04-17
影响因子：
2
通讯作者：
Sedivy, Arthur
中科院分区：
文献类型：
--
作者：
Lopez-Mendez, Blanca;Uebel, Stephan;Sedivy, Arthur
NanoTemper Monolith仪器在学术界和工业界都获得了测量分子相互作用的巨大普及。基础技术已经被广泛地回顾了沿着其假设、限制和应用（Scheuermann等人，Anal Biochem 496：79-93，2016）。关于该技术的几个假设，如由红外激光器产生的热偏差的程度和测量信号的热泳基础已在过去十年中进行了修订。我们在这封信中介绍了在科学服务设施中收集的关于这种技术的经验，并使科学家意识到可能存在的陷阱，目的是在整个科学界推广知识和良好做法。
NanoTemper Monolith instruments have gained enormous popularity for measuring molecular interactions both in academia and industry. The underlying technology has been extensively reviewed along with its assumptions, limitations, and applications (Scheuermann et al., Anal Biochem 496:79-93, 2016). Several assumptions about the technique such as the extent of thermal deviations generated by the infrared laser and the thermophoretic foundation of the measured signal have been revised during the last decade. We present here in this letter the experience gathered in scientific service facilities about this technique and make scientists aware of possible pitfalls with the intention to promote knowledge and good practice throughout the scientific community.