Microinjection of melanin concentrating hormone into the lateral preoptic area promotes non-REM sleep in the rat
Microinjection of melanin concentrating hormone into the lateral preoptic area promotes non-REM sleep in the rat
复制标题
DOI：
10.1016/j.peptides.2012.10.005
复制
发表时间：
2013-01-01
期刊：
影响因子：
3
通讯作者：
Torterolo, Pablo
中科院分区：
文献类型：
--
作者：
Benedetto, Luciana;Rodriguez-Servetti, Zulma;Torterolo, Pablo
腹外侧视前区(VLPO)被认为是产生非REM(NREM)睡眠的关键结构之一。黑色素浓缩激素(MCH)神经元位于下丘脑外侧区和下丘脑腹侧区，广泛投射于整个中枢神经系统，包括向VLPO的投射。MCH与摄食和能量平衡的中枢调节有关。此外，最近的研究结果有力地表明，MCHerEnergy系统促进睡眠。本研究的目的是确定MCH是否通过调节VLPO神经元活动来产生睡眠。为此，我们研究了单侧和双侧VLPO内微量注射MCH对大鼠睡眠和觉醒的影响。单侧向VLPO和邻近的背侧视前区注射MCH并不影响睡眠。相反，与对照(生理盐水)微量注射相比，双侧双侧脑区微量注射MCH显著增加了浅睡眠(LS，39.2+/-4.8vs.21.6+/-2.5min，P<；0.05)和总非快速眼动睡眠(142.4+/-23.2vs.86.5+/-10.5min，P<；0.05)。未观察到对REM睡眠的影响。我们的结论是，在VLPO和邻近的背外侧视前区应用MCH促进了NREM睡眠的产生。(C)2012 Elsevier Inc.保留所有权利。
The ventrolateral preoptic area (VLPO) has been recognized as one of the key structures responsible for the generation of non-REM (NREM) sleep. The melanin-concentrating hormone (MCH)-containing neurons, which are located in the lateral hypothalamus and incerto-hypothalamic area, project widely throughout the central nervous system and include projections to the VLPO. The MCH has been associated with the central regulation of feeding and energy homeostasis. In addition, recent findings strongly suggest that the MCHergic system promotes sleep. The aim of the present study was to determine if MCH generates sleep by regulating VLPO neuronal activity. To this purpose, we characterized the effect of unilateral and bilateral microinjections of MCH into the VLPO on sleep and wakefulness in the rat. Unilateral administration of MCH into the VLPO and adjacent dorsal preoptic area did not modify sleep. On the contrary, bilateral microinjections of MCH (100 ng) into these areas significantly increased light sleep (LS, 39.2 +/- 4.8 vs. 21.6 +/- 2.5 min, P < 0.05) and total NREM sleep (142.4 +/- 23.2 vs. 86.5 +/- 10.5 min, P < 0.05) compared to control (saline) microinjections. No effect was observed on REM sleep. We conclude that MCH administration into the VLPO and adjacent dorsal lateral preoptic area promotes the generation of NREM sleep. (C) 2012 Elsevier Inc. All rights reserved.