The physiological basis for genetic variation in water use efficiency and carbon isotope composition in Arabidopsis thaliana.
The physiological basis for genetic variation in water use efficiency and carbon isotope composition in Arabidopsis thaliana.
复制标题
拟南芥中用水效率和碳同位素组成的遗传变异的生理基础。
DOI：
10.1007/s11120-013-9891-5
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发表时间：
2014-02
影响因子：
3.7
通讯作者：
McKay, John K.
中科院分区：
文献类型：
--
作者：
Easlon, Hsien Ming;Nemali, Krishna S.;Richards, James H.;Hanson, David T.;Juenger, Thomas E.;McKay, John K.
生态学家和生理学家已经记录了拟南芥水分利用效率（WUE）的广泛变化，以及WUE与气候变化的关联。在此，我们展示了全株蒸腾效率和碳同位素组成（δ 13 C）之间的相关性之间的生活史类的A。thaliana.我们还使用整株试管来检查WUE和δ 13 C组成性状的共变模式。我们发现气孔导度（gs）比A更能解释水分利用效率的变化。总的来说，有一个很强的遗传相关性之间的A和GS，符合选择这些性状的比例。在一个更详细的水平上，遗传变异的A是由于潜在的变化，在最大速率的羧化（V cmax）和最大电子传递速率（Jmax）。我们还发现了强烈的影响，叶片解剖，低WUE行有较高的叶片含水量（LWC）和比叶面积（SLA），这表明叶肉导度（克米）的WUE的变化中的作用。我们假设这是由于gm的作用，并使用abi 4突变体来检验这一假设。我们发现ABI4突变体比野生型具有更高的SLA，LWC和g m，与叶片解剖结构的变化导致g m和δ 13 C的变化一致。这些功能数据也进一步支持了ABI4在同时改变阿坝敏感性、糖信号和CO2同化中的中心整合作用。总之，我们的研究结果强调了在功能研究中需要一种更全面的方法，既要更准确地注释基因功能，又要了解植物生长和生产力的共同限制。
Ecologists and physiologists have documented extensive variation in water use efficiency (WUE) in Arabidopsis thaliana, as well as association of WUE with climatic variation. Here, we demonstrate correlations of whole-plant transpiration efficiency and carbon isotope composition (δ13C) among life history classes of A. thaliana. We also use a whole-plant cuvette to examine patterns of co-variation in component traits of WUE and δ13C. We find that stomatal conductance (g s) explains more variation in WUE than does A. Overall, there was a strong genetic correlation between A and g s, consistent with selection acting on the ratio of these traits. At a more detailed level, genetic variation in A was due to underlying variation in both maximal rate of carboxylation (V cmax) and maximum electron transport rate (Jmax). We also found strong effects of leaf anatomy, where lines with lower WUE had higher leaf water content (LWC) and specific leaf area (SLA), suggesting a role for mesophyll conductance (g m) in variation of WUE. We hypothesize that this is due to an effect through g m, and test this hypothesis using the abi4 mutant. We show that mutants of ABI4 have higher SLA, LWC, and g m than wild-type, consistent with variation in leaf anatomy causing variation in g m and δ13C. These functional data also add further support to the central, integrative role of ABI4 in simultaneously altering ABA sensitivity, sugar signaling, and CO2 assimilation. Together our results highlight the need for a more holistic approach in functional studies, both for more accurate annotation of gene function and to understand co-limitations to plant growth and productivity.
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影响因子：
6.9
作者：
Comstock, JP
通讯作者：
Comstock, JP
影响因子：
2.3
作者：
CONDON, AG;RICHARDS, RA;FARQUHAR, GD
通讯作者：
FARQUHAR, GD
影响因子：
7.3
作者：
Barbour, Margaret M.;McDowell, Nate G.;Hanson, David T.
通讯作者：
Hanson, David T.
影响因子：
7.2
作者：
FINKELSTEIN, RR
通讯作者：
FINKELSTEIN, RR
影响因子：
7.3
作者：
Buckley, TN;Mott, KA
通讯作者：
Mott, KA