para-Aminothiophenol Radical Reaction-Functionalized Gold Nanoprobe for One-to-All Detection of Five Reactive Oxygen Species In Vivo
para-Aminothiophenol Radical Reaction-Functionalized Gold Nanoprobe for One-to-All Detection of Five Reactive Oxygen Species In Vivo
复制标题
对氨基苯硫酚自由基反应功能化金纳米探针可一对一检测体内五种活性氧
DOI：
10.1021/acs.analchem.8b03116
复制
发表时间：
2018-10-16
影响因子：
7.4
通讯作者：
Xiao, Zeyu
中科院分区：
文献类型：
--
作者：
Cui, Kai;Fan, Chenchen;Xiao, Zeyu
疾病中产生的五种主要活性氧（ROS）包括H2 O2、（OH）-O-中心点、O-2（中心点-）、ROO中心点和O-1（2）。用单一探针同时检测五种ROS对于全面了解许多疾病的发展和进展至关重要，例如癌症和炎性疾病。然而，目前报道的检测系统受到用一种探针靶向一种ROS的限制。这种一对一的检测模式可能无法充分揭示疾病状态。在这项研究中，我们实现了用一种探针同时检测所有五种ROS（即，一对所有检测），通过设计一种新的对氨基苯硫酚（PATP）和氯化血红素修饰的金（Au/PATP/Hemin）纳米探针。我们发现PATP可以通过自由基氧化偶联机制与（OH）-O-中心点、O-2（中心点-）、ROO中心点和O-1（2）反应生成4，4 '-二巯基偶氮苯（DMAB）。然后DMAB在1142、1386和1432 cm（-1）处引发强的特征表面增强拉曼散射（Sers）峰;这反过来使得能够直接检测（OH）-O-中心点、O-2（中心点-）、ROO中心点和O-1（2），并通过氯化血红素催化的芬顿反应将H2 O2转化为（OH）-O-中心点来间接检测H2 O2。在两种代表性的ROS升高的肿瘤和过敏性皮炎小鼠模型中，Au/PATP/Hemin纳米探针显示出其以高度灵敏和定量的方式监测肿瘤发展和炎症进展的稳健性能。
Five major reactive oxygen species (ROS) are generated in diseases including H2O2, (OH)-O-center dot, O-2(center dot-), ROO center dot, and O-1(2). Simultaneous detection of the five ROS with a single probe is crucial for a comprehensive understanding of the development and progression of many diseases, such as cancer and inflammatory diseases. However, currently reported detection systems are limited by targeting one ROS with one probe. This one-to-one detection mode may fail to sufficiently unveil the diseased state. In this study, we achieved simultaneous detection of all the five ROS with one probe (i.e., one-to-all detection), by designing a novel para-aminothiophenol (PATP) and hemin-decorated gold (Au/PATP/Hemin) nanoprobe. The design is principled by our discovery that PATP can react with (OH)-O-center dot, O-2(center dot-), ROO center dot, and O-1(2) by a radical oxidative coupling mechanism to form 4,4'-dimercaptoazobenzene (DMAB). The DMAB then elicited strong characteristic surface-enhanced Raman scattering (SERS) peaks at 1142, 1386, and 1432 cm(-1); which in turn enables direct detection of (OH)-O-center dot, O-2(center dot-), ROO center dot, and O-1(2) and indirect detection of H2O2 by hemin-catalyzed fenton reaction to convert H2O2 into (OH)-O-center dot. In two representative ROS-elevated mice models of tumors and allergic dermatitis, the Au/PATP/Hemin nanoprobe demonstrated its robust performance of monitoring tumor development and inflammation progression in a highly sensitive and quantitative manner.