Enhancing and Mitigating Radiolytic Damage to Soft Matter in Aqueous Phase Liquid-Cell Transmission Electron Microscopy in the Presence of Gold Nanoparticle Sensitizers or Isopropanol Scavengers
Enhancing and Mitigating Radiolytic Damage to Soft Matter in Aqueous Phase Liquid-Cell Transmission Electron Microscopy in the Presence of Gold Nanoparticle Sensitizers or Isopropanol Scavengers
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DOI：
10.1021/acs.nanolett.0c04636
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发表时间：
2021-01-15
期刊：
影响因子：
10.8
通讯作者：
Gianneschi, Nathan C.
中科院分区：
文献类型：
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作者：
Korpanty, Joanna;Parent, Lucas R.;Gianneschi, Nathan C.
在这项工作中，我们描述了在液体细胞透射电子显微镜（LCTEM）在水中的聚合物所经历的辐解环境。我们研究了在敏化金纳米颗粒（GNP，100 nm）或自由基清除异丙醇（IPA）的存在下，聚（乙二醇）（PEG，2400 g/mol）的水溶液的辐解环境。为了量化聚合物损伤，我们采用了通过基质辅助激光解吸/电离成像质谱（MALDI-IMS）的尸检分析。该方法证实IPA（1- 10%w/v）可以显著减轻辐射裂解诱导的对水中聚合物的损伤，而GNP显著增强损伤。我们耦合LCTEM实验模拟评估辐解缓解或增强提供了一个可推广的策略。这项研究强调了对无机纳米粒子进行LCTEM实验所需的谨慎，其中周围有机材料或溶剂本身的溶液相性质正在调查中。此外，我们预计各种LCTEM研究中清除剂的使用会增加。
In this work, we describe the radiolytic environment experienced by a polymer in water during liquid-cell transmission electron microscopy (LCTEM). We examined the radiolytic environment of aqueous solutions of poly(ethylene glycol) (PEG, 2400 g/mol) in the presence of sensitizing gold nano-particles (GNPs, 100 nm) or radical scavenging isopropanol (IPA). To quantify polymer damage, we employed post-mortem analysis via matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). This approach confirms IPA (1-10% w/v) can significantly mitigate radiolysis-induced damage to polymers in water, while GNPs significantly enhance damage. We couple LCTEM experiments with simulations to provide a generalizable strategy for assessing radiolysis mitigation or enhancement. This study highlights the caution required for LCTEM experiments on inorganic nanoparticles where solution phase properties of surrounding organic materials or the solvent itself are under investigation. Furthermore, we anticipate an increased use of scavengers for LCTEM studies of all kinds.