Vitamin D regulates immunoglobulin mucin domain molecule-4 expression in dendritic cells.
Vitamin D regulates immunoglobulin mucin domain molecule-4 expression in dendritic cells.
复制标题
维生素 D 调节树突状细胞中免疫球蛋白粘蛋白结构域分子 4 的表达。
DOI：
10.1111/cea.12894
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发表时间：
2017
影响因子：
6.1
通讯作者：
Yang P-C
中科院分区：
文献类型：
--
作者：
Liu Z-Q;Li M-G;Geng X-R;Liu J;Yang G;Qiu S-Q;Liu Z-G;Yang P-C
树突状细胞(DC)来源的免疫球蛋白结构域分子(TIM)4在启动辅助性T细胞(Th)2极化过程中起着关键作用。维生素D(VitD)参与多种免疫反应的调节。目的验证维生素D调节DC TIM4表达的假说。方法采集变应性鼻炎(AR)患者和健康人外周血标本。结果AR患者血清中维生素D3的活性形式骨化三醇水平明显低于正常对照组。外周DC高表达TIM4，低表达VDR。DC血清骨化三醇与TIM4呈负相关。培养中加入骨化三醇的DC可增加VDR的表达。我们还发现VDR与树突状细胞TIM4启动子结合，抑制了TIM4基因的转录和表达。结论维生素D缺乏可能通过增加TIM4的表达参与AR的发病。提示AR的治疗可能需要调节血清骨化三醇水平和DC中VDR的表达。
BackgroundDendritic cell (DC)‐derived immunoglobulin domain molecule (TIM)4 plays a critical role in the initiation of T helper (Th)2 polarization. Vitamin D (VitD) involves the regulation of a number of immune responses.ObjectivesThis study tests a hypothesis that VitD regulates TIM4 expression in DCs.MethodsPeripheral blood samples were collected from patients with allergic rhinitis (AR) and healthy subjects. DCs were isolated from the samples and analyzed for the expression of TIM4.ResultsWe observed that the levels of calcitriol, the active form of VitD3, in the sera of AR patients were lower than that in healthy subjects. The peripheral DC expressed higher levels of TIM4 and lower levels of VDR. A negative correlation was identified between the data of serum calcitriol and TIM4 in DCs. Exposure DCs to calcitriol in the culture increased the expression of VDR. We also found that VDR bound to the TIM4 promoter locus in DCs to repress the TIM4 gene transcription and expression.Conclusions and clinical relevanceVitD deficiency may contribute to the pathogenesis of AR by increasing the TIM4 expression. The results suggest that to regulate the serum calcitriol levels and the expression of VDR in DCs may be necessary to be taken into account in the treatment of AR.