Schwann Cell But Not Olfactory Ensheathing Glia Transplants Improve Hindlimb Locomotor Performance in the Moderately Contused Adult Rat Thoracic Spinal Cord
Schwann Cell But Not Olfactory Ensheathing Glia Transplants Improve Hindlimb Locomotor Performance in the Moderately Contused Adult Rat Thoracic Spinal Cord
复制标题
DOI：
10.1523/jneurosci.22-15-06670.2002
复制
发表时间：
2002-08
期刊：
影响因子：
--
通讯作者：
T. Takami;M. Oudega;Margaret L. Bates;P. Wood;N. Kleitman;M. Bunge
中科院分区：
文献类型：
--
作者：
T. Takami;M. Oudega;Margaret L. Bates;P. Wood;N. Kleitman;M. Bunge
在中度挫伤（10 gm, 12.5 mm， NYU撞击器）1周后，将培养的成年大鼠雪旺细胞（SCs）或嗅鞘胶质细胞（OEG）或两者同时移植到成年费舍尔大鼠胸（T9）脊髓中。大鼠分别接受2 × 106个细胞悬浮于培养基或只接受培养基（对照组）。损伤后12周，所有移植动物均出现空化现象。虽然在中剂量注射的大鼠中，在损伤部位中心的5毫米长的脊髓段内，有33%的脊髓组织被保留，但在SC（51%）、OEG（43%）和SC/OEG（44%）移植动物中，保留的组织明显更多。所有三种类型的神经胶质移植物都充满轴突，主要来自脊柱。SC移植物比SC/OEG和OEG移植物含有更多的髓鞘轴突。两种含sc的移植物与仅含oeg的移植物相比，胶质纤维酸性蛋白和硫酸软骨素蛋白多糖的染色更强烈。逆行示踪显示，与对照组相比，SC和SC/OEG移植的本体脊髓和脑干轴突的数量明显高于移植区域外5-6 mm，而仅OEG移植的数量明显高于对照组。与对照组相比，所有移植动物的皮质脊髓纤维末端更靠近病灶中心。仅sc移植后，损伤后8-11周后肢运动能力有适度但有统计学意义的改善。因此，除了这种功能改善之外，我们的研究结果表明，在中度挫伤的成年大鼠胸脊髓中，SC移植比SC/OEG或OEG移植更有效地促进轴突保留/再生。
Cultured adult rat Schwann cells (SCs) or olfactory ensheathing glia (OEG), or both, were transplanted in the adult Fischer rat thoracic (T9) spinal cord 1 week after a moderate contusion (10 gm, 12.5 mm, NYU impactor). Rats received either a total of 2 × 106 cells suspended in culture medium or culture medium only (controls). At 12 weeks after injury, all grafted animals exhibited diminished cavitation. Although in medium-injected rats 33% of spinal tissue within a 5-mm-long segment of cord centered at the injury site was spared, significantly more tissue was spared in SC (51%), OEG (43%), and SC/OEG (44%) grafted animals. All three types of glial grafts were filled with axons, primarily of spinal origin. SC grafts contained more myelinated axons than SC/OEG and OEG grafts. Both types of SC-containing grafts expressed more intense staining for glial fibrillary acidic protein and chondroitin sulfate proteoglycan compared with OEG-only grafts. Retrograde tracing demonstrated that the number of propriospinal and brainstem axons reaching 5–6 mm beyond the grafted area was significantly higher with SC and SC/OEG grafts but not with OEG-only grafts compared with controls. Corticospinal fibers terminated closer to the lesion epicenter in all grafted animals than in controls. With SC-only grafts, a modest but statistically significant improvement in hindlimb locomotor performance was detected at 8–11 weeks after injury. Thus, in addition to this functional improvement, our results show that an SC graft is more effective in promoting axonal sparing/regeneration than an SC/OEG or OEG graft in the moderately contused adult rat thoracic spinal cord.