Polyphasic characterization of benthic, moderately halophilic, moderately thermophilic cyanobacteria with very thin trichomes and the proposal of Halomicronema excentricum gen. nov., sp nov.
Polyphasic characterization of benthic, moderately halophilic, moderately thermophilic cyanobacteria with very thin trichomes and the proposal of Halomicronema excentricum gen. nov., sp nov.
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DOI：
10.1007/s00203-001-0390-2
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发表时间：
2002-05-01
影响因子：
2.8
通讯作者：
Hernández-Mariné, M
中科院分区：
文献类型：
--
作者：
Abed, RMM;Garcia-Pichel, F;Hernández-Mariné, M
描述了一个具有极薄毛状体的中度嗜盐、中度耐盐和中度嗜热蓝藻新属。从人工高盐池塘的底栖微生物席中分离得到该属的4株菌。毛状体约1妈妈厚，在横壁和分流无色鞘小收缩。类囊体平行于细胞壁，但类囊体和类核通常在细胞质内相对于毛状体主轴偏心排列。菌株生长在3.2和12-15%（w/v）的盐度之间，最适盐度在3.2和12%之间。它们显示温度下限在20摄氏度左右，上限在45到50摄氏度之间，最佳温度在28到45- 50摄氏度之间。菌株之间的类胡萝卜素和菌孢菌素氨基酸补充物是相同的。基于16 S rRNA基因序列的系统发育分析表明，所有菌株的密切相关（99%或更高的相似性）和远亲其他蓝藻（91%或更低的相似性）。我们提出了新的属和种Halomwicronema excentricum为这些菌株。模式菌株为TFEP 1。
A new genus of moderately halophilic, moderately halotolerant and moderately thermophilic cyanobacteria with very thin trichomes is described. The four strains included in this genus were isolated from benthic microbial mats in a man-made hypersaline pond. Trichomes were around 1 mum thick, with small constrictions at the cross-walls and diffluent colorless sheaths. Thylakoids were parallel to the cell wall, but thylakoids and nucleoid were often excentrically arranged within the cytoplasm with respect to the main trichome axis. Strains grew at between 3.2 and 12-15% (w/v) salinity with optima between 3.2 and 12%. They showed lower temperature limits around 20degreesC and upper limits between 45 and 50degreesC, with optima between 28 and 45-50degreesC. Carotenoid and mycosporine amino-acid complements were identical among strains. Phylogenetic analyses based on 16S rRNA gene sequence showed that all strains were closely related (99% or higher similarity) and distantly related to other cyanobacteria (91% or lower similarity). We propose the new genus and species Halomwicronema excentricum for these strains. The type strain is TFEP1.