Isolation of type 5 adenovirus mutants with a cold-sensitive host range phenotype: genetic evidence of an adenovirus transformation maintenance function.
Isolation of type 5 adenovirus mutants with a cold-sensitive host range phenotype: genetic evidence of an adenovirus transformation maintenance function.
复制标题
具有冷敏感宿主范围表型的 5 型腺病毒突变体的分离：腺病毒转化维持功能的遗传证据。
DOI：
10.1016/0042-6822(82)90381-6
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发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
Williams,J
中科院分区：
文献类型：
--
作者：
Ho,YS;Galos,R;Williams,J
我们已经分离到5型腺病毒的宿主范围突变体，它们具有冷敏感的宿主范围表型。这些突变株在32.5°和38.5°温度下在293细胞上生长良好，在38.5°温度下在HeLa细胞上生长良好，但在低温下对HeLa细胞生长抑制。与5型原生突变体的互补和重组试验将这些新突变体归入组I，定位于早期区域1A(ELA)，组II，定位于早期区域1B(ELB)。Ela突变体具有冷敏转化表型，转化和转化细胞表型的温移分析结果提供了强有力的遗传证据，表明E1a基因的功能直接或间接地需要维持转化。ELB突变体在高温和低温下都是有效的转化缺陷，但在38.5°建立的转化子表现出冷敏感的表型。因此，ELB基因的功能可能同时参与启动和维持。
We have isolated host range mutants of type 5 adenovirus which possess a cold-sensitive host range phenotype. These mutants grow well on 293 cells at 32.5° and 38.5°, and on HeLa cells at 38.5° but show restricted growth upon HeLa cells at the low temperature. Complementation and recombination tests with prototypehranddlmutants of type 5 assign these new mutants to group I, mapping in early region 1A (ElA), and group II, mapping in early region 1B (ElB). The ElA mutants possess a cold-sensitive transformation phenotype, and the results of temperature-shift analyses of transformation and transformed cell phenotype provide strong genetic evidence that an E1A gene function is required either directly or indirectly for maintenance of transformation. The ElB mutants are effectively transformation defective at both high and low temperatures, buthrtransformants established at 38.5° do show a cold-sensitive phenotype. Thus, an ElB gene function may be involved in both initiation and maintenance.