Correlation between membrane expansion and temperature-induced membrane fusion.
Correlation between membrane expansion and temperature-induced membrane fusion.
复制标题
膜膨胀与温度诱导的膜融合之间的相关性。
DOI：
10.1016/0005-2736(81)90452-1
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Ohki,S
中科院分区：
文献类型：
--
作者：
Chaudhury,MK;Ohki,S
对于每个磷脂膜，有一个特征性的相变温度，对于每个磷脂球形膜，有一个特定的“融合”温度。为了研究温度诱导的膜融合和膜膨胀之间可能的相关性，磷脂膜在两种温度下的物理状态之间的关系进行了研究通过使用的单层系统。单层膨胀研究表明，由从相转变到融合温度的温度增加引起的每个脂质分子面积的增加对于所使用的五种不同磷脂大致相同。在相同的温度升高下，含有胆固醇的磷脂单分子膜没有明显的膨胀。这与定性相关的膜融合的更大的抑制中看到的球形磷脂膜系统时，胆固醇被纳入membrane.The磷脂酰丝氨酸单分子膜的膨胀的pH值的影响也进行了研究有关膜融合现象。球形磷脂膜的融合温度的变化，由于pH值的变化被解释为在脂质膜的相变温度的变化。从相变温度增加到熔合温度引起的单层中每个分子的膨胀面积是大致相同的，而与表面电荷密度无关。
For each phospholipid membrane, there is a characteristic phase transition temperature, and for each phospholipid spherical membrane, there is a specific ‘fusion’ temperature. In order to examine the possible correlation between temperature-induced membrane fusion and membrane expansion, the relationship between the physical states of phospholipid membranes at both temperatures have been investigated by the use of the monolayer system. Monolayer expansion studies have indicated that the increase in area per lipid molecule, caused by increasing the temperature from the phase transition to the fusion temperature, is approximately the same for five different phospholipids used. With the same temperature increase, phospholipid monolayers containing cholesterol did not expand appreciably. This correlates qualitatively with the greater inhibition of membrane fusion seen in the spherical phospholipid membrane systems when cholesterol was incorporated in the membrane.The effect of pH on the expansion of phosphatidylserine monolayers was also studied in relation to membrane fusion phenomena. The shift in fusion temperature of the spherical phospholipid membranes due to the change of pH is explained by the shift in phase transition temperatures of lipid membranes. The expanded area per molecule in the monolayer caused by increasing the temperature from the phase transition to the fusion temperature was approximately the same irrespective of surface charge densities.