Environmental Enrichment Increases Radiation-induced Apoptosis Not Spontaneous Apoptosis in Mouse Intestinal Crypt Cells
Environmental Enrichment Increases Radiation-induced Apoptosis Not Spontaneous Apoptosis in Mouse Intestinal Crypt Cells
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DOI：
10.21873/invivo.12745
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发表时间：
2022
期刊：
影响因子：
2.3
通讯作者：
Shinya Yokomizo;M. Nishimura;T. Morioka;Utako Enzaka;C. Tsuruoka;Y. Shang;Y. Nishimura;Kazumasa Inoue;M. Fukushi;T. Imaoka;S. Kakinuma;Y. Shimada
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文献类型：
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作者：
Shinya Yokomizo;M. Nishimura;T. Morioka;Utako Enzaka;C. Tsuruoka;Y. Shang;Y. Nishimura;Kazumasa Inoue;M. Fukushi;T. Imaoka;S. Kakinuma;Y. Shimada
背景/目的：丰富的环境(EE)改变小鼠中枢神经系统(CNS)中细胞的凋亡和促进细胞增殖。然而，很少有研究考察EE对模型器官中非中枢器官细胞凋亡的影响。此外，肠道是辐射后致癌的高危器官之一。在此，我们评估了EE对自发和辐射诱导的小鼠肠隐窝细胞凋亡的影响。材料和方法：幼龄(3周龄)和成年(11周龄)雄性B6C3F1小鼠在标准环境或EE中饲养8周，然后接受2GyX射线全身照射。结果：EE能显著降低体重、脂肪组织重量及血清总胆固醇、甘油三酯、瘦素和胰岛素水平。虽然EE不改变照射后结肠隐窝自发凋亡指数，但能显著增加照射后结肠隐窝自发凋亡指数。小肠隐窝的细胞凋亡指数也表现出类似的模式。结论：EE可增强辐射诱导的小肠和结肠干/祖细胞的凋亡，而不影响自发的细胞凋亡。因此，EE可能会降低放射治疗等辐射暴露后患肠道癌症的风险。
Background/Aim: An enriched environment (EE) modifies apoptotic cell death and promotes cell proliferation in the central nervous system (CNS) in mice. However, few studies have examined the effects of an EE on apoptosis in non-CNS organs in model orgamisms. In addition, the intestinal tract is one of organs at high-risk of carcinogenesis after radiation exposure. Herein we evaluated the effects of an EE on spontaneous and radiation-induced apoptosis in intestinal crypt cells of mice. Materials and Methods: Juvenile (3-week-old) and adult (11-week-old) male B6C3F1 mice were housed in a standard environment or EE for 8 weeks and then were whole-body irradiated with 2 Gy X-rays. Apoptosis in the small intestine and colon was analyzed with antibody against cleaved caspase 3. Results: The EE significantly reduced body weight; adipose tissue weight; and serum levels of total cholesterol, triglyceride, leptin, and insulin. Although EE did not change the spontaneous apoptotic index without irradiation, it significantly increased the index after irradiation in the colonic crypt. The apoptotic index in the small intestinal crypt showed similar patterns. Conclusion: An EE enhances radiation-induced apoptosis of stem/progenitor cells in the small intestine and colon without affecting spontaneous apoptosis. An EE may thus reduce the risk of cancer in the intestinal tract after radiation exposure such as radiotherapy.